Syed Faisal Badshah1, Naveed Akhtar1, Muhammad Usman Minhas2, Kifayat Ullah Khan1, Samiullah Khan3, Orva Abdullah4, Abid Naeem5. 1. Faculty of Pharmacy and Alternative Medicine, the Islamia University of Bahawalpur, Punjab, Pakistan. 2. College of Pharmacy, University of Sargodha, University Road Sargodha City, Punjab, Pakistan. Electronic address: us.minhas@hotmail.com. 3. Department of Pharmacy, The University of Lahore, Gujrat Campus, Pakistan. 4. Hamdard Institute of Pharmaceutical Sciences, Hamdard University, Islamabad, Pakistan. 5. Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330004, China.
Abstract
AIMS: Aim of the study was to enhance the solubility of Chlorthalidone by developing beta-cyclodextrin cross-linked hydrophilic nanomatrices. MAIN METHODS: Nine different formulations were fabricated by free radical polymerization technique. All formulations were characterized through different studies. FTIR spectroscopy of unloaded and loaded nanomatrices was processed to determine compatibility of constituents and that of the drug with the system. Surface morphology of the nanomatrices was studied by SEM. The size of the optimized formulation was determined by zeta sizer. Swelling, in-vitro release and solubility studies were carried out in different media and results of in-vitro release profiles of nanomatrices and commercially available tablet of Chlorthalidone were compared. For determination of biocompatibility, toxicity studies were proclaimed in rabbits. KEY FINDINGS: Main peaks of corresponding functional groups of individual constituents and that of drug were depicted in FTIR spectra of unloaded and loaded nanomatrices. Porous and fluffy structure was visualized through SEM images. Particle size of the optimized formulation was in the range of 175 ± 5.27 nm. Percent loading of optimized formulation showed the best result. Comparing the in-vitro drug release profiles of nanomatrices and commercially available tablet, the results of the synthesized nanomatrices were quite satisfactory. Solubility profiles were also high as compared to the drug alone. Moreover, toxicity studies confirmed that nanomatrices were biocompatible and no sign of any toxic effect was found. SIGNIFICANCE: We concluded that our developed nanomatrices had successfully enhanced the solubility of Chlorthalidone and can also be used for other poorly aqueous soluble drugs.
AIMS: Aim of the study was to enhance the solubility of Chlorthalidone by developing beta-cyclodextrincross-linked hydrophilic nanomatrices. MAIN METHODS: Nine different formulations were fabricated by free radical polymerization technique. All formulations were characterized through different studies. FTIR spectroscopy of unloaded and loaded nanomatrices was processed to determine compatibility of constituents and that of the drug with the system. Surface morphology of the nanomatrices was studied by SEM. The size of the optimized formulation was determined by zeta sizer. Swelling, in-vitro release and solubility studies were carried out in different media and results of in-vitro release profiles of nanomatrices and commercially available tablet of Chlorthalidone were compared. For determination of biocompatibility, toxicity studies were proclaimed in rabbits. KEY FINDINGS: Main peaks of corresponding functional groups of individual constituents and that of drug were depicted in FTIR spectra of unloaded and loaded nanomatrices. Porous and fluffy structure was visualized through SEM images. Particle size of the optimized formulation was in the range of 175 ± 5.27 nm. Percent loading of optimized formulation showed the best result. Comparing the in-vitro drug release profiles of nanomatrices and commercially available tablet, the results of the synthesized nanomatrices were quite satisfactory. Solubility profiles were also high as compared to the drug alone. Moreover, toxicity studies confirmed that nanomatrices were biocompatible and no sign of any toxic effect was found. SIGNIFICANCE: We concluded that our developed nanomatrices had successfully enhanced the solubility of Chlorthalidone and can also be used for other poorly aqueous soluble drugs.
Authors: Aousaf Ahmad; Mahmood Ahmad; Muhammad Usman Minhas; Muhammad Sarfraz; Muhammad Sohail; Kifayat Ullah Khan; Sana Tanveer; Shakeel Ijaz Journal: Biomed Res Int Date: 2022-07-19 Impact factor: 3.246