Elizabeth Loredo-García1, Alejandra Ortiz-Dosal2, Juan Manuel Núñez-Leyva2, José Luis Cuellar Camacho3, Jorge Alejandro Alegría-Torres4, Lizeth García-Torres5, Hugo Ricardo Navarro-Contreras1, Eleazar Samuel Kolosovas-Machuca1. 1. Coordinación para la Innovación y Aplicación de la Ciencia y la Tecnología, Universidad Autónoma de San Luis Potosí. 550 Sierra Leona Ave., 78210 San Luis Potosí, SLP, México. 2. Doctorado Institucional en Ingeniería y Ciencia de Materiales (DICIM-UASLP), Universidad Autónoma de San Luis Potosí. 550 Sierra Leona Ave., 78210 San Luis Potosí, SLP, México. 3. Institute of Chemistry & Biochemistry, Freie Universität Berlin, Takustraße 3, 14195 Berlin, Germany. 4. Departamento de Farmacia, División de Ciencias Naturales y Exactas, Universidad de Guanajuato. Noria Alta s/n, 36050, Guanajuato, Gto., México. 5. Laboratorio de Investigación Molecular en Nutrición (LIMON), Universidad del Centro de México, UCEM, Capitán Caldera 75, 78250, San Luis Potosí, SLP, México.
Abstract
Background: TNF-α is a cytokine involved in inflammation. Surface-enhanced Raman spectroscopy (SERS) could be useful in its detection. Aim: Identify the TNF-α in an aqueous solution, using gold nanoparticles (AuNPs) as a SERS substrate. Materials & methods: Raman and SERS spectra were obtained from TNF-α samples, combined with AuNPs, with decreasing concentrations of TNF-α. The samples were analyzed using optical transmission spectroscopy, dynamic light scattering, and transmission electron microscopy. Results: Transmission electron microscopy/dynamic light scattering determined a change in the average diameter of the TNF-α/AuNPs (∼9.6 nm). Raman bands obtained were associated with aromatic amino acid side chains. We observe Raman signals for TNF-α concentrations as low as 0.125 pg/ml. Conclusion: TNF-α signal at physiological concentrations was determined with SERS.
Background: TNF-α is a cytokine involved in inflammation. Surface-enhanced Raman spectroscopy (SERS) could be useful in its detection. Aim: Identify the TNF-α in an aqueous solution, using gold nanoparticles (AuNPs) as a SERS substrate. Materials & methods: Raman and SERS spectra were obtained from TNF-α samples, combined with AuNPs, with decreasing concentrations of TNF-α. The samples were analyzed using optical transmission spectroscopy, dynamic light scattering, and transmission electron microscopy. Results: Transmission electron microscopy/dynamic light scattering determined a change in the average diameter of the TNF-α/AuNPs (∼9.6 nm). Raman bands obtained were associated with aromatic amino acid side chains. We observe Raman signals for TNF-α concentrations as low as 0.125 pg/ml. Conclusion: TNF-α signal at physiological concentrations was determined with SERS.
Authors: Juan Manuel Núñez-Leyva; Eleazar Samuel Kolosovas-Machuca; John Sánchez; Edgar Guevara; Alexander Cuadrado; Javier Alda; Francisco Javier González Journal: Nanomaterials (Basel) Date: 2021-06-28 Impact factor: 5.076