Simultaneous analytical efficiency evaluation using HPTLC method for analysis of syringicacid and vanillic acid and their anti-oxidant capacity from methanol extract of Ricinus communis Linn. and Euphorbia hirta Linn.

BACKGROUND: Well-known medicinal plants Ricinus communis Linn. and Euphorbia hirta Linn. have been traditionally used to treat a variety of ailments in the India and other countries.
OBJECTIVE: In present research, a simple, rapid, reliable and accessible HPTLC technique has been established for concurrent quantification of phenolic compounds (syringic acid and vanillic acid)and evaluation of their anti-oxidant capacity.
METHOD: The chromatographic separation was conceded on pre-coated silica gel plates F254 as the stationary phase. The appropriate mobilephasecombination toluene: ethyl acetate: formic acid (7:2.5:0.5 v/v/v) were developed to expand the plates which separated components according to the marker compounds. Further, the anti-oxidant potential of methanol extract R. communis (MERC) and E. hirta (MERH) were also assessed with (DPPH) by using UV spectrophotometer.
RESULTS: Densitometric scanning was performed by Camag scanner V and measured at two different wave length 272 nm and 318 nm. The marker compounds were practically resolved with RF 0.5 ± 0.04 for syringic acid and 0.6 ± 0.06 for vanillic acid. The results obtained in the study of anti-oxidant activity of methanol extract R. communis(MERC) and E. hirta (MERH) were showed significant free radical scavenging capacity against DPPH generated free radicals.
CONCLUSIONS: The developed HPTLC method was validated for accuracy, linearity, precision, and specificity. Both the extract has revealed considerable antioxidant activity. The reported existing phenolic and flavonoids compounds are responsible for antioxidant activity of plant extract. © AOAC INTERNATIONAL 2020. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.