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Literature DB >> 333401

Structure of chromatin subunits: an endonuclease Serratia marcescens study.

V A Pospelov, S B Svetlikova, V I Vorob'ev.

Abstract

Electrophoretic properties of chromatin subunits--nucleosomes--obtained by treatment of chromatin with the Serratia marcescens endonuclease have been studied. Double-stranded breaks of DNA between adjacent nucleosomes do not necessarily lead to their disjunction. Fragmentation of the DNA within the nucleosomes may proceed simultaneously with the breakdown of the DNA between the nucleosomes at early stages of the endonuclease digestion. Electrophoretic mobility and chromatographic properties of mononucleosomes, their dimers and trimers with internally degraded DNA was not changed. It has been deduced that the integrity of chromatin particles with internally fragmented DNA is supported by histone interaction inside and between the nucleosomes.

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Year: 1977 PMID： 333401 PMCID： PMC342649 DOI： 10.1093/nar/4.9.3267

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

17 in total

4. Nucleosomal structure of sea urchin and starfish sperm chromatin. Histone H2B is possibly involved in determining the length of linker DNA.

Authors: I A Zalenskaya; V A Pospelov; A O Zalensky; V I Vorob'ev
Journal: Nucleic Acids Res Date: 1981-02-11 Impact factor: 16.971

4 in total

Structure of chromatin subunits: an endonuclease Serratia marcescens study.

Review 1. Chromosomal proteins and chromatin structure.

2. Nuclease cleavage of chromatin at 100-nucleotide pair intervals.

3. Dissociation and reconstitution of chromatin without appreciable degradation of the proteins.

4. Spheroid chromatin units (v bodies).

5. Specific sites of interaction between histones and DNA in chromatin.

6. Chromatin structure: a repeating unit of histones and DNA.

7. Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease.

8. An extracellular nuclease from Serratia marcescens. I. Purification and some properties of the enzyme.

9. Heterogeneity of chromatin subunits.

10. Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis.

1. Nucleosome packing in chromatin as revealed by nuclease digestion.

2. X-ray small-angle scattering study of mononucleosomes and of the close packing of nucleosomes in polynucleosomes.

3. DNA degradation in isolated rat liver nuclei.

4. Nucleosomal structure of sea urchin and starfish sperm chromatin. Histone H2B is possibly involved in determining the length of linker DNA.