Huanjun Chen1, Weizheng Zhou2, Shunni Zhu2, Fen Liu1, Lei Qin2, Chao Xu3, Zhongming Wang4. 1. Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China; CAS Key Laboratory of Renewable Energy, Guangzhou 510640, China; Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, China; University of China Academy of Sciences, Beijing 100049, China. 2. Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China; CAS Key Laboratory of Renewable Energy, Guangzhou 510640, China; Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, China. 3. Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China. 4. Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China; CAS Key Laboratory of Renewable Energy, Guangzhou 510640, China; Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, China. Electronic address: wangzm@ms.giec.ac.cn.
Abstract
Strain C-13, identified as an Acinetobacter sp. by homology searches, exhibited efficient simultaneous heterotrophic nitrification-aerobic denitrification phosphorus removal (SNDPR) abilities by nitrogen balance analysis and further confirmation of successful amplification of functional genes ppk, napA, and nirS. In addition, strain C-13 could utilize NH4+-N, NO3--N, and NO2--N as nitrogen sources, among which NH4+-N was indicated to be an excellent nitrogen source for assimilation and heterotrophic nitrification. Besides, the optimum conditions for nutrient removal were determined as follows: sodium acetate as the sole carbon source, C/N/P ratio of 100/10/2, pH = 7.5, and temperature of 30 °C. Meanwhile, the strain also showed the traditional features, such as release and the excess uptake of phosphate under anaerobic/aerobic conditions, with the highest phosphorus content of 5.01% after cultivation. Strain C-13 presents promising prospects for application in biologicalnutrient removal in wastewater treatment.
Strain C-13, identified as an class="Species">Acinetobacter sp. by homology searches, exhibited efficieclass="Chemical">nt simultaclass="Chemical">neous heterotrophic class="Chemical">nitrificatioclass="Chemical">n-aerobic declass="Chemical">nitrificatioclass="Chemical">n class="Chemical">n class="Chemical">phosphorus removal (SNDPR) abilities by nitrogen balance analysis and further confirmation of successful amplification of functional genes ppk, napA, and nirS. In addition, strain C-13 could utilize NH4+-N, NO3--N, and NO2--N as nitrogen sources, among which NH4+-N was indicated to be an excellent nitrogen source for assimilation and heterotrophic nitrification. Besides, the optimum conditions for nutrient removal were determined as follows: sodium acetate as the sole carbon source, C/N/P ratio of 100/10/2, pH = 7.5, and temperature of 30 °C. Meanwhile, the strain also showed the traditional features, such as release and the excess uptake of phosphate under anaerobic/aerobic conditions, with the highest phosphorus content of 5.01% after cultivation. Strain C-13 presents promising prospects for application in biologicalnutrient removal in wastewater treatment.