Literature DB >> 33336838

Color tuning of fluorogens for FAST fluorogen-activating protein.

Ivan N Myasnyanko1, Alexey S Gavrikov1, Snizhana O Zaitseva2, Alexander Yu Smirnov1, Elvira R Zaitseva2, Anatolii I Sokolov1, Kseniya K Malyshevskaya1, Nadezhda S Baleeva1, Alexander S Mishin2, Mikhail S Baranov3.   

Abstract

Using benzylidene imidazolone core, we created a panel of color-shifted fluorogenic ligands for FAST protein without compromise to the binding efficiency and the utility for live-cell protein labeling. This study highlights the potential of benzylidene imidazolones derivatives for rapid expansion of a pallet of live-cell fluorogenic labeling tools.
© 2020 Wiley-VCH GmbH.

Entities:  

Keywords:  Chromophore; GFP; fluorescence; fluorescent labelling; fluorogen-activating protein

Year:  2020        PMID： 33336838     DOI： 10.1002/chem.202004760

Source DB:  PubMed          Journal:  Chemistry        ISSN： 0947-6539            Impact factor:   5.236


  4 in total

1.  The Fluorescence-Activating and Absorption-Shifting Tag (FAST) Enables Live-Cell Fluorescence Imaging of Methanococcus maripaludis.

Authors:  Eric Hernandez; Kyle C Costa
Journal:  J Bacteriol       Date:  2022-06-03       Impact factor: 3.476

Review 2.  Transient Fluorescence Labeling: Low Affinity-High Benefits.

Authors:  Maxim M Perfilov; Alexey S Gavrikov; Konstantin A Lukyanov; Alexander S Mishin
Journal:  Int J Mol Sci       Date:  2021-10-30       Impact factor: 5.923

3.  Structure-based rational design of an enhanced fluorogen-activating protein for fluorogens based on GFP chromophore.

Authors:  Marina V Goncharuk; Nadezhda S Baleeva; Dmitry E Nolde; Alexey S Gavrikov; Alexey V Mishin; Alexander S Mishin; Andrey Y Sosorev; Alexander S Arseniev; Sergey A Goncharuk; Valentin I Borshchevskiy; Roman G Efremov; Konstantin S Mineev; Mikhail S Baranov
Journal:  Commun Biol       Date:  2022-07-15

4.  Spatial Structure of NanoFAST in the Apo State and in Complex with its Fluorogen HBR-DOM2.

Authors:  Vladislav A Lushpa; Nadezhda S Baleeva; Sergey A Goncharuk; Marina V Goncharuk; Alexander S Arseniev; Mikhail S Baranov; Konstantin S Mineev
Journal:  Int J Mol Sci       Date:  2022-09-26       Impact factor: 6.208
  4 in total

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