Hosein Rasekh1, Mehdi Hoseini Farahi1, Davood Mehrabani2,3,4,5, Seyed Jalil Massoumi6, Mani Ramzi7, Jason P Acker5,8. 1. Department of Horticultural Sciences, Yasooj Branch, Islamic Azad University, Yasooj, Iran. 2. Stem Cell Technology Research Center, Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran. 3. Burn and Wound Healing Research Center, Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran. 4. Comparative and Experimental Medicine Center, Shiraz University of Medical Sciences, Shiraz, Iran. 5. Department of Pathology, University of Alberta, Edmonton, AB, Canada. 6. Nutrition and Food Sciences Research Center, Department of Nutrition, School of Nutrition and Food Sciences, Shiraz University of Medical Sciences, Shiraz, Iran. 7. Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 8. Centre for Innovation, Canadian Blood Services, Edmonton, AB, Canada.
Abstract
BACKGROUND: Feijoa is widely used in medicine due to their anti-inflammatory, antioxidant, antimicrobial and antitumor properties. The current investigation studied the proliferative and regenerative effect of acetonic extract of Feijoa sellowiana on stem cells. METHODS: Acetone extract of Feijoa was prepared using percolator and rotary machines. Human bone marrow stem cells (hBMSCs) were used as experimental in vitro model and characterized morphologically, by flowcytometry, and differentiation properties. The toxicity of the extract on hBMSCs was determined by MTT assay. The viability and growth kinetics of hBMSCs treated to Feijoa was determined. Real time PCR was used for changes in expression of proliferative and apoptotic genes on day 7th. RESULTS: MTT assay demondtrated that Feijoa at doses less than 200 ng/ml did not show any cytotoxic effect on hBMSCs and increased the cell proliferation until day 3rd followed by a non-significant slow decreasing trend until day 7th. Population doubling time (PDT) showed a decline until day 3rd followed by an increase until day 7th. A significant rise in expression of Bax and decline in Bcl-2 expression were noted on day 7th. CONCLUSION: The modulatory activity of Feijoa may be responsible for its increasing effect on cell proliferation till day 3rd. Therefore, when faster proliferation during a shorter time period is targeted, Feijoa can be safely added to the culture media in the first three days.
BACKGROUND: Feijoa is widely used in medicine due to their anti-inflammatory, antioxidant, antimicrobial and antitumor properties. The current investigation studied the proliferative and regenerative effect of acetonic extract of Feijoa sellowiana on stem cells. METHODS:Acetone extract of Feijoa was prepared using percolator and rotary machines. Human bone marrow stem cells (hBMSCs) were used as experimental in vitro model and characterized morphologically, by flowcytometry, and differentiation properties. The toxicity of the extract on hBMSCs was determined by MTT assay. The viability and growth kinetics of hBMSCs treated to Feijoa was determined. Real time PCR was used for changes in expression of proliferative and apoptotic genes on day 7th. RESULTS:MTT assay demondtrated that Feijoa at doses less than 200 ng/ml did not show any cytotoxic effect on hBMSCs and increased the cell proliferation until day 3rd followed by a non-significant slow decreasing trend until day 7th. Population doubling time (PDT) showed a decline until day 3rd followed by an increase until day 7th. A significant rise in expression of Bax and decline in Bcl-2 expression were noted on day 7th. CONCLUSION: The modulatory activity of Feijoa may be responsible for its increasing effect on cell proliferation till day 3rd. Therefore, when faster proliferation during a shorter time period is targeted, Feijoa can be safely added to the culture media in the first three days.
Entities:
Keywords:
Apoptosis; Bone marrow stem cells; Feijoa sellowiana; Growth kinetics
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