Association of Low Leptin with Poor 3-Month Prognosis in Ischemic Stroke Patients with Type 2 Diabetes.

BACKGROUND: Leptin, an adipokine, has effects on the cardiovascular system with both protective and harmful role. This study aimed to assess the relationship between leptin and 3-month prognosis in ischemic stroke patients with type 2 diabetes. PATIENTS AND METHODS: As a prospective single-center observational study, we collected consecutive first-ever acute ischemia stroke with type 2 diabetes mellitus from February 2019 to February 2020. Serum samples were obtained at admission, and leptin serum levels were tested by the ELISA method. Logistic regression models were used to assess leptin's prognostic value to predict the functional outcome and mortality within three months.
RESULTS: Finally, two hundred and eleven patients were included, and the mean leptin serum level was 16.8 (SD. 6.9) ng/mL. At admission, 53.6% of those included patients (N=113) were defined as severe stroke (NIH Stroke Scale [NIHSS]>5). In multivariable models adjusted for other factors, leptin levels<11.6ng/mL (lowest quartile, Q1) related to severe stroke and the risk increased 175% (odds ratios [OR] =2.75; 95% confidence interval [CI]=2.13-3.38; P=0.002). Serum leptin levels on admission in patients with poor outcomes and nonsurvivors were significantly reduced (P<0.001 and P<0.001). Leptin levels <11.6ng/mL (lowest quartile, Q1) related to a higher risk of poor functional impairment (OR=5.13; 95% CI =3.25-6.86; P<0.001) and mortality (OR=3.19; 95% CI =2.03-4.25; P<0.001).
CONCLUSION: The data shows that leptin serum level is a useful prognostic biomarker in ischemic stroke patients with type 2 diabetes, and this relationship is negative.

Background

Leptin was identified in 1994 as an adipocyte-derived signaling factor, which, after interaction with its receptors, played an intricate role in body weight and energy expenditure.1 Furthermore, leptin plays a role in the regulation of neuroendocrine and immune function.2 Loffreda et al3 showed that leptin played a role in the up‐regulation of inflammatory immune responses, contributing to several significant obesity complications.

Plasma leptin levels correlate strongly with diabetes4 and metabolic syndrome.5 Leptin receptors could express in the cardiovascular system,6 and some studies found that hyperleptinemia had effects on obesity-associated cardiovascular diseases and atherosclerosis.7 Knudson et al8 showed that hyperleptinemia could produce significant coronary endothelial dysfunction. In addition to its role in anti-obesity, leptin also presented antidiabetic actions.9 In patients with type 2 diabetes, Reilly et al10 reported that leptin plasma levels were related to coronary artery calcification.

The leptin role in predicting carotid intima-media thickness, carotid plaque instability, and cerebrovascular disease was proposed.11,12 However, the association between leptin and stroke was not confirmed in the other two studies.13,14 Furthermore, one study showed that serum level of leptin was related to lesion size and stroke severity,15 while another study reported that serum leptin level at discharge could predict post-stroke depression development during the next month.16 A review suggested that leptin’s blood level was a useful prognostic biomarker for inflammation and atherosclerosis in stroke.17 However, the relationship between leptin and functional impairment in ischemic stroke with type 2 diabetes is still not well understood. Thus, this study aimed to assess the relationship between leptin and 3-month prognosis in ischemic stroke patients with type 2 diabetes.

Patients and Methods

As a prospective single-center observational study, we collected consecutive first-ever acute ischemia stroke (AIS) with type 2 diabetes mellitus from February 2019 to February 2020. Those patients were from the Department of Emergency of our hospital and presented with symptom onset within 48 hours. AIS and diabetes were defined according to the World Health Organization criteria.18,19 Patients with tumors, metabolic syndrome, intracerebral hemorrhage, liver, renal insufficiency, systemic infections, and autoimmune diseases were excluded.

One hundred age and sex-matched healthy volunteers from our hospital’s physical examination center were assigned to the healthy control group. The mean age was 67.5 years, and 42.0% were women. The Ethics Committee of the Nanchang University Third Hospital reviewed and approved the trial protocol according to the Declaration of Helsinki (1964). Written informed consent was obtained from patients before entering the study. We confirmed that any data intended for sharing would be de-identified.

MRI confirmed the clinical diagnoses of stroke within 24 hours after admission, and Dr. Li determined diffusion-weighted imaging (DWI) lesion volumes according to the formula 0.5×a×b×c (specific information could be found in the literature).20 Ischemic strokes were classified according to the original Trial of Org 10,172 in Acute Stroke Treatment (TOAST) criteria.21 National Institutes of Health Stroke Scale (NIHSS) was used to assess stroke’s clinical severity on admission.22 A severe stroke was defined as an NIHSS of more than 5.

Within 24 hours after admission, some information was recorded: vital signs; medication before stroke (hypertension, hypercholesterolemia, atrial fibrillation, cardiovascular diseases, peripheral vascular disease, and transient ischemic attack); IV thrombolysis and mechanical thrombectomy as acute stroke treatment; common factors (ie, age; sex; body index mass [BMI], smoking history; duration of diabetes; insulin and/or hypoglycemic agents as diabetes treatment). Fasting blood samples from all participants were collected within the first 24 h after admission, and serum samples were separated and used to test leptin levels by a commercially available quantitative enzyme-linked immunosorbent assay (ELISA) kit (Abcam, China). In this study, the detection range was from 4.0ng/mL to 400.0ng/mL, and the intra and inter-assay coefficient of variation [CV] was 5.2–8.3% and 5.8%-9.2%, respectively.

We conducted a 3-month follow-up by a structured telephone interview. The functional impairment was assessed, and a poor functional impairment was defined as a modified Rankin scale (mRS) score of 3 to 6 points, while a good outcome was described as an mRS score of 0 to 2 points.23 Besides, death events and the causes of death during follow-up were recorded.

Statistical Analysis

Data results were recorded as categorical variables (number [%]) and continuous variables (means [Standard deviation, SD]). Results between different groups were compared by the chi-square and unpaired T-test when appropriate.

Associations between leptin serum level and either severe stroke, poor functional impairment, or mortality at follow-up were analyzed using logistic regression models. The results were presented as odds ratios (OR) and 95% confidence intervals (CI). Serum leptin levels were categorized into quartiles, and the lowest quartile (Q1) was compared to the other three quartiles (Q2-Q4, as the reference). In multivariate models, we included all variables with a p-value <0.05 in unadjusted models.

Lastly, the optimal threshold of serum level of leptin in diagnosing either high clinical severity reduced functional impairment, or mortality was assessed by the receiver operating characteristic (ROC) curves to obtain the area under the curve (AUC) and 95% CI. Significance was set at P<0.05, and all statistical analysis was assessed by the SPSS22.0 software (SPSS Inc, Chicago, USA).

Data Sharing

Please contact the corresponding author for all data requests.

Results

As shown in Figure 1, 408 ischemia stroke patients with diabetes were screened, and finally, 211 patients were included and analyzed. However, the baseline characteristics [age (P=0.11), gender (P=0.51), NIHSS (P=0.17), and BMI (P=0.63)] in those included patients were similar to the screened patients. Those patients’ mean age was 67.3 (SD. 11.3) years, and 57.3% were male. The leptin serum level was obtained with a mean value of 16.8 (SD. 6.9) ng/mL, and the median value was 16.3ng/mL (IQR, 11.6–21.3). The mean serum level of leptin in AIS was significantly lower than in those control cases (16.8[SD:6.8] vs 18.5 [7.2] ng/mL; P<0.001). The serum levels of leptin were not related to the time from stroke symptom onset to blood collected (P=0.38). The baseline characteristics of those 211 included cases are presented in Table 1.

Figure 1

Study profile/flow sheet of the study.

Table 1

Baseline Characteristics of Stroke Patients with Diabetes‡

Characteristics	Total	Low Leptin	Normal Leptin	P‡
N	211	54	157	–
Age, years mean (±SD)	67.3 (11.3)	66.9 (11.3)	67.4 (11.4)	0.86
<60	58 (27.5)	16 (29.6)	42 (26.8)	–
60–74	82 (38.9)	21 (38.9)	61 (38.9)	–
≥75	71 (33.6)	17 (31.5)	54 (34.4)	–
Male gender	121 (57.3)	36 (66.7)	85 (54.1)	0.38
BMI, kg/m2 mean (±SD)	24.4 (3.7)	22.8 (2.6)	24.9 (3.7)	0.43
Duration of diabetes, years mean (±SD)	11.5 (2.8)	13.8 (3.0)	11.1 (2.7)	<0.001
Intensive glucose treatment†	75 (35.5)	23 (42.6)	52 (33.1)	0.209
Vascular risk factors
Hypertension	127 (60.2)	35 (64.8)	92 (58.6)	0.421
Hypercholesterolemia	62 (29.4)	18 (33.3)	44 (28.0)	0.460
Atrial fibrillation	31 (14.7)	8 (14.8)	23 (14.6)	0.976
CVD	45 (21.3)	15 (27.8)	30 (19.1)	0.180
TIA	22 (10.4)	10 (18.5)	12 (7.6)	0.024
Smoking	41 (19.4)	12 (22.2)	29 (18.5)	0.550
Peripheral vascular disease	9 (4.3)	2 (3.7)	7 (4.5)	0.225
Stroke etiology				0.104
Small-vessel occlusive	42 (19.9)	12 (22.2)	30 (19.1)	–
Large-vessel occlusive	44 (20.9)	11 (20.3)	33 (21.0)	–
Cardioembolic	73 (34.6)	20 (37.0)	53 (33.8)	–
Other	21 (10.0)	8 (14.8)	13 (12.1)	–
Unknown	31 (14.7)	3 (5.6)	28 (17.8)	–
Acute treatment
IV thrombolysis	25 (11.8)	5 (9.3)	20 (12.7)	0.495
Mechanical thrombectomy	8 (3.8)	3 (5.6)	5 (3.2)	0.431
IV thrombolysis and/or Mechanical thrombectomy	30 (14.2)	6 (11.1)	24 (15.3)	0.449
NIHSS at admission, mean (±SD)	7.7 (4.7)	10.4 (4.5)	6.8 (4.5)	<0.001
NIHSS <6	98 (46.4)	13 (24.1)	85 (54.1)	<0.001
NIHSS ≥6	113 (53.6)	41 (75.9)	72 (45.9)
Lesion volumes at admission, mL mean (±SD)	15.3 (13.5)	18.7 (14.9)	14.1 (12.8)	0.031
Hospital stay, days mean (±SD)	13.5 (5.7)	14.4 (6.0)	13.2 (5.6)	0.096
Hospital costs, Yuan mean (±SD)	12,553 (2422)	13,764 (3743)	12,675 (2419)	0.535
Serum levels of biomarkers at admission, mean (±SD)
Leptin, ng/mL	16.8 (6.9)	8.9 (1.9)	19.5 (5.8)	<0.001
FSG, mmol/l	6.63 (1.94)	6.82 (2.37)	6.02 (1.77)	0.233
CRP, mg/l	4.5 (2.1)	6.6 (3.1)	4.1 (2.0)	0.002
Rankin at 3-month median (IQR)	1 (0–3)	4 (3–5)	1 (0–2)
mRS 0–2	133 (63.0)	13 (24.1)	120 (76.4)	<0.001
mRS 3–6	78 (37.0)	41 (75.9)	37 (23.6)	–
Mortality at 3-month				<0.001
Survivors	179 (84.8)	35 (64.8)	144 (91.7)	–
Nonsurvivors	32 (15.2)	19 (35.2)	13 (8.3)	–

Notes: ‡Results are expressed as number (%) or as mean (SD). Results between different groups were compared by the chi-square and unpaired T-test when appropriate. Low serum leptin level was defined as the lowest quartile (Q1) <11.6ng/mL. †Sulfonylurea or insulin or, if more than 120% of ideal body weight, metformin.

Abbreviations: BMI, body mass index; SD, standard deviation; CVD, cardiovascular diseases; TIA, transient ischemic attack; IV, intravenous; NIHSS, the NIH Stoke Scale; FSG, fasting serum glucose; CRP, C reaction proteins; mRS, modified Rankin scale.

At admission, 53.6% of those included patients (N=113) were defined as severe stroke. The mean leptin serum level was lower in those patients than in those patients with minor clinical severity (15.7[SD, 7.0]ng/mL vs 18.0[6.5]ng/mL, P=0.014), Figure 2A. In multivariable models adjusted for other factors, leptin levels<11.6ng/mL (lowest quartile, Q1) related to severe stroke and the risk increased 175% (odds ratios [OR] =2.75; 95% confidence interval [CI]=2.13–3.38; P=0.002). As shown in Figure 2B, the cut-off value of serum leptin in diagnosing severe stroke was estimated to be 12.0 ng/mL with the AUC (95% CI) of 0.61 (0.53–0.68), which showed that the sensitivity and specificity were 86.7% and 38.9%, respectively.

Figure 2

The association between serum leptin and stroke severity at admission. (A) Serum level of leptin in patients with minor stroke and severe stroke. The data presented as mean ± SD. (B) Receiver operating characteristic (ROC) curve was utilized to evaluate the accuracy of the serum level of leptin to diagnose severe stroke.

At 3-month, 133 patients (63.0%) had good functional impairment whereas 78 patients (37.0%) had unfavorable functional impairment. The mean leptin serum level was lower in the latter group than that observed in patients with good functional impairment (12.6[SD. 5.1]ng/mL vs 19.3[6.6] ng/mL, P<0.001) (Figure 3A). The data showed that 41 out of 54 patients (75.9%) with low serum leptin (<11.6ng/mL) on admission developed poor outcomes, while 37 out of 157 patients (23.6%) with normal serum leptin (<11.6ng/mL) on admission developed poor outcomes. In a multivariate analysis model, serum leptin levels<11.6ng/mL (lowest quartile, Q1) related to a higher risk of poor functional outcome (OR=5.13; 95% CI =3.25–6.86; P<0.001) (Table 2). Furthermore, the association between poor outcome and leptin was more pronounced among men (OR [95% CI]: 5.65[3.77–7.32]) when compared with women (4.95[3.01–6.63]). As shown in Figure 3B, the cut-off value of serum leptin in diagnosing poor functional impairment was estimated to be 13.3 ng/mL with the AUC (95% CI) of 0.81 (0.74–0.87), which suggested that the sensitivity and specificity were 74.0% and 80.0%, respectively.

Figure 3

The association between serum leptin and stroke outcome at 3-month. (A) Serum level of leptin in patients with poor functional outcome and good functional outcome. The data presented as a mean ± SD. (B) Receiver operating characteristic (ROC) curve was utilized to evaluate the accuracy of leptin’s serum level to poor functional outcomes. A modified Rankin scale (mRS) score of 3 to 6 points was defined as a poor functional outcome.

Table 2

Multivariate Analysis of Predictors of Poor Functional Outcome and Mortality†

	OR	95% CI	P
Functiona2l outcome
Leptin <11.6 ng/mL‡	5.13	3.25–6.86	<0.001
NIHSS >5	7.15	4.09–10.23	<0.001
Acute treatment	0.36	0.30–0.42	<0.001
CRP (per unit increase)	1.21	1.12–1.35	0.006
Lesion volumes (per unit increase)	1.15	1.05–1.27	0.013
Duration of diabetes	2.03	1.35–2.69	0.019
Mortality
Leptin <11.6 ng/mL‡	3.19	2.03–4.25	<0.001
NIHSS >5	8.29	3.65–12.44	<0.001
Acute treatment	0.27	0.21–0.34	<0.001
CRP (per unit increase)	1.28	1.10–1.40	0.005
Lesion volumes (per unit increase)	1.17	1.03–1.30	0.011

Notes: †Adjusted for age, gender, BMI, duration of diabetes, diabetes treatment, medication before stroke (hypertension, hypercholesterolemia, atrial fibrillation, cardiovascular diseases, peripheral vascular disease, and transient ischemic attack), NIHSS at admission, stroke subtype, and acute stroke treatment. ‡Serum levels of leptin were categorized into quartiles, and the lowest quartiles (Q1=11.6ng/mL) were compared to the other three quartiles (as the reference).

At 3-month, the mortality rate was 15.2% (N=32). The mean leptin serum level in those patients was lower than in those survivors (10.8[SD. 4.4]ng/mL vs 17.8[6.7] ng/mL, P<0.001) (Figure 4A). The data showed that 19 out of 54 patients (35.2%) with low serum leptin (<11.6ng/mL) on admission were nonsurvivors, while 13 out of 157 patients (8.3%) with normal serum leptin (<11.6ng/mL) on admission were nonsurvivors. In a multivariate analysis model, serum leptin levels<11.6ng/mL (lowest quartile, Q1) related to a higher risk of mortality (OR=3.19; 95% CI =2.03–4.25; P<0.001) (Table 2). As shown in Figure 4B, the cut-off value of serum leptin in diagnosing mortality was estimated to be 13.0 ng/mL with the AUC (95% CI) of 0.82 (0.74–0.89), which suggested that the sensitivity and specificity were 73.5% and 79.3%, respectively.

Figure 4

The association between serum leptin and stroke mortality at 3-month. (A) Serum level of leptin in survivors and nonsurvivors. The data presented as mean ± SD. (B) Receiver operating characteristic (ROC) curve was utilized to evaluate the accuracy of the serum level of leptin to mortality.

Discussion

To our knowledge, we firstly assessed the prognostic value of leptin serum levels in ischemic stroke patients with diabetes. The data shows that leptin serum level is a useful prognostic biomarker in ischemic stroke patients with type 2 diabetes, and this relationship is negative, suggesting a neuroprotective role in stroke patients.

As an adipocyte-secreted hormone, leptin affects neuroendocrine function,24 and the neuroprotective role against ischemic neuronal injury has been proposed.25 The neuroprotection role of leptin during cerebral ischemia had been suggested by one study,25 while another study showed that exogenous leptin administration could protect against ischemic neuronal injury.26 Furthermore, Zhang et al27 demonstrated that leptin’s neuroprotective role might be related to the increase of Calcitonin gene-related peptide expression, while another study suggested that leptin-induced neuroprotection mainly through the PI3K/Akt pathway.28 In addition, Zhang et al29 reported that leptin could protect hippocampal CA1 neurons against ischemic injury through ERK1/2 MAPK signaling pathways. At the same time, another suggested that leptin might be used to attenuate ischemic injury after stroke via the induction of an anti-apoptotic state.30

One study also suggested that leptin’s benefit after stroke was related to its new neuroprotective role.31 Furthermore, the role of leptin in glycaemic control had been suggested,32 and the prognostic value of glucose serum level on admission in stroke patients treated with thrombolysis had been proposed.33 Also, low adiponectin/leptin could lead to increased inflammation response and oxidative stress.34

As an observational study, we could not obtain a causal relationship between leptin and stroke prognosis. Whether stroke patients with low serum leptin levels need to supplement leptin to maintain optimal concentrations to improve stroke prognosis requires further human trials. Interestingly, one study showed that a combined application of leptin and tissue plasminogen activator could improve stroke prognosis.35 However, Avraham et al31 found that leptin-induced neurogenesis and angiogenesis could not contribute to the functional outcome improvement,31 while another study reported that elevated leptin plasma level was related to larger infarct volume following tissue plasminogen activator treatment.36 Therefore, the therapeutic role of leptin in stroke patients needs further research to verify.

Consistent with our findings, Ku et al37 showed that low leptin level was related to a high risk of cardiovascular events and mortality in stable coronary artery disease patients. At the same time, another study illuminated that higher leptin/adiponectin at day one was associated with better functional outcomes in atherothrombotic stroke patients.38 Furthermore, another study reported that higher baseline leptin level was a protective factor of cardiovascular events (including strokes).39 However, conflicting results exist with a study reporting high plasma leptin levels to predict major adverse cardiac events in coronary artery disease patients independently of established risk factors.40 Liu et al15 also showed that increasing levels of serum leptin correlated with stroke severity and lesion size. This discrepancy might be caused by the differences in the research population and testing methods. Furthermore, another study found that plasma leptin was an independent predictor of recurrent cardiovascular events in men with earlier acute coronary syndromes.41 Similarly, one study showed that leptin was an independent positive predictor of future cardiovascular events in patients with angiographically confirmed coronary atherosclerosis,42 and Wallander et al43 showed that high serum levels of leptin were associated with a poorer long-term prognosis in patients with myocardial infarction and without type 2 diabetes mellitus. Another study did not find any significant association between serum leptin levels and the risk of cardiovascular diseases or all-cause mortality in women with diabetes.44

Leptin is an adipokine have effects on the cardiovascular system with both protective and harmful role. The above studies assessing the relationship between leptin and prognosis had yielded conflicting results, which might be explained by the obesity paradox.

Clinical Respective

In ischemic stroke patients with diabetes, low leptin serum levels at admission were independently associated with a poor functional prognosis, suggesting that leptin could be used for attenuating ischemic injury and improving prognosis after stroke.

Study Limitation

This study included limitations. First, this was a single-center observational study with small samples (N=211). Besides, it impossible to determine causality associations from this study. Second, we only collected serum samples. The levels of cerebrospinal fluid leptin were not tested. One study showed that leptin could cross the blood-brain barrier and was also synthesized in the brain.35 Third, other adipokine biomarkers were not tested. The association of those factors with leptin and stroke prognosis could not be determined.

Conclusions

The data shows that leptin serum level is a useful prognostic biomarker in ischemic stroke patients with type 2 diabetes, and this relationship is negative. This association is also independent of other factors, including gender and obesity.