Huey-Chun Huang1, Shr-Shiuan Wang2, Tsang-Chi Tsai3, Wang-Ping Ko3, Tsong-Min Chang2. 1. Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung City 406040, Taiwan. 2. Department of Applied Cosmetology, HungKuang University, Taichung City 43302, Taiwan. 3. O'right Plant Extract R&D Center, Hair O'right International Corporation, Taoyuan City 32544, Taiwan.
Abstract
BACKGROUND: The mode of action of Phoenix dactylifera seed extract in skin care has never been explored. METHODS: P. dactylifera L. seeds were extracted by ultrasonic extraction. The antioxidant characteristics of the extract were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-di-(3-ethylbenzthiazoline sulfonic acid) (ABTS+) assays and scavenging methods. The total phenolic content, reducing capacity, iron (II) ion-chelation, and intracellular reactive oxygen species (ROS)-scavenging capacities were also investigated. The effects of P. dactylifera L. seed extract on melanogenesis were evaluated spectrophotometrically by a mushroom tyrosinase activity assay, determination of intracellular tyrosinase activity, and melanin content. The expression levels of melanogenesis-related proteins were analyzed by Western blotting. RESULTS: The results revealed that the P. dactylifera L. seed extract exerted apparent antioxidant capacity and significantly decreased intracellular ROS content at concentrations of 0.245 and 0.49 (mg/mL). Furthermore, the extract decreased the expression of melanocortin 1 receptor (MC1R), microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein-1 (TRP1), and tyrosinase-related protein-2 (TRP2), and inhibited melanogenesis in B16F10 cells. CONCLUSIONS: Our results revealed that P. dactylifera L. seed extract attenuated melanogenesis in B16F10 cells by downregulating protein kinase A (PKA) signaling pathways. Hence, the extract could be used as a type of skin-whitening agent in skin care products.
BACKGROUND:The mode of action ofPhoenix dactyliferaseed extract in skin care has never been explored. METHODS:P. dactylifera L. seeds were extracted by ultrasonic extraction. The antioxidant characteristics oftheextract were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-di-(3-ethylbenzthiazoline sulfonic acid) (ABTS+) assays and scavenging methods. The total phenolic content, reducing capacity, iron (II) ion-chelation, and intracellular reactive oxygen species (ROS)-scavenging capacities were also investigated. The effects of P. dactylifera L. seed extract on melanogenesis were evaluated spectrophotometrically by a mushroomtyrosinase activity assay, determination of intracellular tyrosinase activity, and melanin content. The expression levels of melanogenesis-related proteins were analyzed by Western blotting. RESULTS:The results revealed that the P. dactylifera L. seed extract exerted apparent antioxidant capacity and significantly decreased intracellular ROS content at concentrations of 0.245 and 0.49 (mg/mL). Furthermore, theextract decreased the expression ofmelanocortin 1 receptor (MC1R), microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein-1 (TRP1), and tyrosinase-related protein-2 (TRP2), and inhibited melanogenesis in B16F10 cells. CONCLUSIONS: Our results revealed that P. dactylifera L. seed extract attenuated melanogenesis in B16F10 cells by downregulating protein kinase A (PKA) signaling pathways. Hence, theextract could be used as a type of skin-whitening agent in skin care products.
Authors: Zulhisyam Abdul Kari; Mahmoud A O Dawood; Nik Shahman Nik Ahmad Ariff; Muhammad Rajaei Ahmad Mohd Zain; Zeti Norfidiyati Salmuna; Norzila Ismail; Al Hafiz Ibrahim; Kumara Thevan Krishnan; Nor Fazila Che Mat; Hisham Atan Edinur; Mohammad Khairul Azhar Abdul Razab; Aurifullah Mohammed; Sirajudeen Kuttulebbai Naina Mohamed Salam; Pasupuleti Visweswara Rao; Sakinah Mohamad; Basyarah Hamat; Shahriman Zainal Abidin; Lee Seong Wei; Amran Ahmed Shokri Journal: Appl Biochem Biotechnol Date: 2022-05-17 Impact factor: 3.094