| Literature DB >> 33326084 |
Xi Cheng1, Samuel T Peters2, Shondra M Pruett-Miller2, Thomas L Saunders3, Bina Joe4.
Abstract
The CRISPR/Cas9 system has been widely used as an efficient genome-editing tool for studying physiological functions of long noncoding RNAs (lncRNAs). In this chapter, we describe the experimental procedures for using the CRISPR/Cas9 system to genetically modify a long noncoding RNA in vivo through the targeted disruption and knockin approaches.Keywords: CRISPR/Cas9; Disruption; In vivo; Knockin; lncRNA
Year: 2021 PMID： 33326084 DOI： 10.1007/978-1-0716-1158-6_19
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745