Literature DB >> 33326081

M(R)apping RNA-Protein Interactions.

Jasmine Barra1,2, Roberto Vendramin1,2, Eleonora Leucci3.   

Abstract

The systematic investigation of RNA-protein interactions is a key step towards a better understanding of the functions of RNA molecules.We developed an easy-to-use method to isolate and identify RNAs and proteins bound to long non-coding RNAs (lncRNAs ) in their native configuration. Similar to other methodologies, we utilize biotinylated antisense oligonucleotides (ASOs) to purify the lncRNA of interest and its associated proteins from different cellular compartments.

Entities:  

Keywords:  Mass spectrometry; Proteins; RAP; Subcellular fractions; lncRNAs

Year:  2021        PMID： 33326081     DOI： 10.1007/978-1-0716-1158-6_16

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

1.  Genomic maps of long noncoding RNA occupancy reveal principles of RNA-chromatin interactions.

Authors:  Ci Chu; Kun Qu; Franklin L Zhong; Steven E Artandi; Howard Y Chang
Journal:  Mol Cell       Date:  2011-09-29       Impact factor: 17.970

Review 2.  Mass spectrometry approaches to study mammalian kinase and phosphatase associated proteins.

Authors:  Michelle J Kean; Amber L Couzens; Anne-Claude Gingras
Journal:  Methods       Date:  2012-06-16       Impact factor: 3.608

3.  Melanoma addiction to the long non-coding RNA SAMMSON.

Authors:  Eleonora Leucci; Roberto Vendramin; Marco Spinazzi; Patrick Laurette; Mark Fiers; Jasper Wouters; Enrico Radaelli; Sven Eyckerman; Carina Leonelli; Katrien Vanderheyden; Aljosja Rogiers; Els Hermans; Pieter Baatsen; Stein Aerts; Frederic Amant; Stefan Van Aelst; Joost van den Oord; Bart de Strooper; Irwin Davidson; Denis L J Lafontaine; Kris Gevaert; Jo Vandesompele; Pieter Mestdagh; Jean-Christophe Marine
Journal:  Nature       Date:  2016-03-24       Impact factor: 49.962

Review 4.  Probing Long Non-coding RNA-Protein Interactions.

Authors:  Jasmine Barra; Eleonora Leucci
Journal:  Front Mol Biosci       Date:  2017-07-11

Review 5.  Methods for comprehensive experimental identification of RNA-protein interactions.

Authors:  Colleen A McHugh; Pamela Russell; Mitchell Guttman
Journal:  Genome Biol       Date:  2014-01-27       Impact factor: 13.583
  5 in total

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