Success of 125I-Seed Treatment in Vulvar Squamous-Cell Carcinoma with Aplastic Anemia: A Case Report.

Vulvar squamous-cell carcinoma (SCC) is a rare disease that occurs mainly in postmenopausal women. Chemo/radiotherapy with or without surgery is the most important modality for treatment of advanced vulvar cancer. A case of vulvar SCC with aplastic anemia was treated using 125I seeds in our department, because surgery and chemotherapy were not possible due to low platelets, leaving radiotherapy as the lone therapeutic option. 125I seeds present an alternative option for treatment of patients with vulvar SCC and local relapse with lymph-node metastasis following previous radiotherapy.

Introduction

Vulvar cancer is the fourth–most frequent type of gynecological cancer, accounting for approximately 5% of all female genital tract malignancies.1 According to data from the American Cancer Society, 6,120 cases of vulvar cancer were newly diagnosed and 1,350 women died from this disease in the US in 2020.2 The most common histological type of vulvar cancer is squamous-cell carcinoma (SCC), which accounts for around 90% of cases.3 Surgery is the main treatment for vulvar cancer,4 with radical resection of the vulva and bilateral inguinal lymphadenectomy commonly applied surgical modalities.5 Current standard surgical treatments frequently lead to unsatisfactory outcomes, particularly in patients with node-positive disease.6 Further, chemo/radiotherapy, with or without surgery is the most important treatment modality for advanced vulvar cancer.7 Advanced vulvar SCC has limited treatment options, and recurrence is often lethal.8 Despite the number of treatment options, patients continue to be afflicted by posttreatment side effects, including substantial morbidity, lymphedema, sexual and psychological dysfunction, and wound-healing disorders.9 Moreover, up to 40% of patients with early-stage vulvar SCC develop recurrence within 10 years.10 Unfortunately, not all patients are suitable for surgery and chemotherapy, such as those with aplastic anemia (AA), a type of acquired bone marrow–failure syndrome.11 AA is an immunomediated disease caused by the destruction of hematopoietic cells, resulting in pancytopenia.12

Implantation of iodine 125 (125I) seeds into tumors is a targeted-radiotherapy method. Under the guidance of ultrasound and other imaging techniques, 125I seeds, which emit low-energy gamma rays, are directly implanted into tumors to kill the malignant tissue. As the dose intensity of 125I is inversely proportional to the square of distance, the cumulative dose in normal tissue around the target tumor is minimized.13 This local treatment is safe and effective, and can provide long-term local control of tumors.14 Adjunctive radiotherapy, such as 125I seeds, significantly lowers the risk of recurrence at the site of the primary tumor relative to patients treated without radiotherapy.15 For some patients with recurrence or localized metastasis after surgery, the use of 125I seeds has obvious advantages. 125I seeds combined with chemotherapy and other systemic therapy can also kill distant metastatic lesions, with clear effects on tumor treatment. In addition, this approach can also be used to supplement conventional radiotherapy and as a means of coordinated treatment to achieve better therapeutic effects.16

Here, we report a case of vulvar SCC in a patient with AA who was treated using 125I seeds in our department in recent years.

Case Report

Here, we present the case of a 50-year-old premenopausal woman who had developed painless, slowly growing masses accompanied by irregular bleeding under the skin of the vulva in August 2016. The masses were located in the left labium majus of the vulva, without palpable inguinal lymph nodes bilaterally. One was 3×3 cm and the other 2×2 cm. No ulceration was observed. Laboratory tests (hormone panel, and gynecological and breast cancer markers) were within normal limits. Pathological examination of a vulva biopsy on August 19, 2016 showed moderately and poorly differentiated SCC (Figure 1). A total-body computed-tomography scan and whole-body bone imaging, performed to determine disease stage, were negative for distant metastases.

Figure 1

Histopathologic specimen. (A) H&E staining, 100×. (B) Ki67 staining, 100×. (C) p16 staining, 100×. (D) p63 staining, 100×.

The patient had a medical history of AA of >20 years, and had taken folic acid, androgens, and cyclosporine to control the disease. The specific dose of medication was unknown. However, the drugs were ineffective. Subsequently, she was treated with regular blood transfusions, with platelet counts ≤10×109/L. Further, she had a history of type2 diabetes of >4 years.

Due to the low platelet count of the patient because of AA, surgery and chemotherapy were not possible. Local radiotherapy with DT72GY/36FX was performed from August 24, 2016 using noncoplanar electron-beam perpendicular irradiation with conventional linear accelerator on vulvar masses covered with 0.5 mm wet gauze, and the patient placed in the lithotomy position at a collimator angle of 90º to the linear accelerator. A significant regression of the masses in the vulva was found on subsequent physical examination; however, she had grade II radiation injury of the skin, which is common and unavoidable, despite the use of radioprotective agents. During treatment, platelets and red blood cells were supplemented by occasional component blood transfusion.

In August 2018, the patient palpated a hard mass in the left inguinal groin, with poor mobility and no tenderness, which had gradually increased in the previous 2 months. On pelvic MRI, a mass of approximately 37×35 mm was observed in the left inguinal region (Figure 2). Due to coagulation disorder resulting from AA, a biopsy was not performed. A diagnosis of metastasis in the left inguinal lymph node was considered, based on MRI. Radiotherapy of the left inguinal groin may cause lymphatic reflux disturbance; therefore, the patient was treated with 35 125I seeds in the left inguinal node under the guidance of ultrasound on October 26, 2018. The biologically effective dose of an 125I seed is almost 100 Gy. The 125I seeds were mainly implanted in the center of the mass at a distance of 1 cm or more from the edge to reduce damage to the skin. On November 18, 2018, the left inguinal mass was approximately 33×33 mm on MRI (Figure 3). Therefore, the patient was again treated with eight 125I seeds in the left inguinal node as a supplementary local dose on January 18, 2019.

Figure 2

Mass of 37×35 mm detected in the left inguinal region on pelvic MRI. (A) Low signal intensity of approximately 37×35 mm detected in the left inguinal region on T1-weighted imaging (arrow). (B) High signal intensity of approximately 37×35 mm detected in the left inguinal region on T2-weighted imaging (arrow).

Figure 3

Mass of 33×33 mm detected in the left inguinal region on pelvic MRI. (A) Low signal intensity of approximately 33×33 mm detected in the left inguinal region on T1-weighted imaging (arrow). (B) High signal intensity of approximately 33×33 mm detected in the left inguinal region on T2-weighted imaging (arrow).

On February 22, 2019, the patient had a mass in the vulva. A 21×16 mm mass and a nodular shadow of approximately 9×8 mm were detected in the left inguinal region on pelvic MRI (Figure 4), indicating that the mass in the groin was receding and the particle implantation was effective. Therefore, 5 days later, 125I seeds were implanted into the base of the new masses in the vulva and left inguinal region. The superficial mass in the vulva gradually subsided soon after seed implantation. At the same time, skin ulceration was still appearing. Unfortunately, 2 months later, the patient developed two more palpable lumps: one of 3×3 cm and the other 2×2 cm. Simultaneously, a 15×6 mm mass was detected in the left inguinal region on ultrasound. Therefore, on May 10, 2019, the patient was treated with implantation of 60 125I seeds in the base of the new lump and left inguinal region. After implantation, the mass completely disappeared again. Although the patient developed bleeding of the vulvar mass, it stopped in response to symptomatic treatment (wet wrap with 0.002% norepinephrine daily and 0.6 g ethylenediamine diaceturate intravenously guttae daily). The ulcers of the epidermis remained.

Figure 4

Mass of 21×16 mm and a nodular shadow of approximately 9×8 mm detected in the left inguinal region on pelvic MRI. (A) Low signal intensity of approximately 21×16 mm detected in the left inguinal region on T1-weighted imaging (arrow). (B) High signal intensity of approximately 21×16 mm was detected in the left inguinal region on T2-weighted imaging (arrow). (C) Mild enhancement of a nodular shadow (9×8 mm) after injection of contrast-enhancing agent (circle).

According to data collected at follow-up examinations performed between May 2019 and June 2019, the patient was able to conduct normal activities, and no new lesions were found. Unfortunately, the patient developed a cough and hemoptysis, and multiple metastatic lesions were found in the lungs on July 12, 2019. In August 2019, the patient received radiotherapy with DT45GY/15FX for the lesions in the lungs; however, the cough and hemoptysis were aggravated following DT27GY/9FX radiotherapy. The patient died on October 31, 2019.

Discussion

This was a unique case of vulvar SCC with AA in a 50-year-old premenopausal woman. Vulvar cancer mainly occurs in older women, primarily those who are postmenopausal.17 The peak age of incidence of vulvar cancer has been reported as >70 years; however, the peak period for vulvar cancer among Chinese women is <60 years.18 The patient reported here was a middle-aged premenopausal woman with a history of AA. Surgical treatment is usually the first choice for patients with early-stage vulvar SCC, including radical vulvectomy with bilateral inguinofemoral lymphadenectomy as a treatment of choice for vulvar SCC;19 however, the substantial loss of tissue involved in such surgery results in a risk of postoperative complications.20 After consultation with several hospitals, it was concluded that surgery and chemotherapy would be intolerable for our patient, because of her history of AA. In this case, radiotherapy was the only option. Radiation therapy has been regarded as a highly effective treatment for patients with noninvasive and inoperable vulvar cancer,21 with recurrence rates of 20% and 35% for patients with stage III and IV disease, respectively.22

Vulvar cancer lesions can directly infiltrate adjacent tissue and organs, such as the vagina, urethral orifice, and anus, or spread directly to the opposite side. Further, vulvar cancer can spread from its original site to locoregional nodes. The 5-year overall survival rate for patients with vulvar cancer without nodal metastasis is >90%, while that for patients with nodal metastasis is <60%.23 Our patient was clinically assessed as lymph-node negative at first diagnosis. Unfortunately, she was unable to sustain prolonged disease-free survival, and experienced local relapse and nodal involvement 2 years after the first radiotherapy. Due to the risk of exceeding normal tissue tolerance, doses are often limited for patients with recurrence after previous radiotherapy. Nevertheless, brachytherapy, such as 125I seeds, allows dose escalation in such situations, which can provide a perfectly targeted and localized boost dose and potentially improve patient outcomes.24

For 125I seeds, a physical dose of 100 Gy delivered at an initial dose rate of approximately 0.05 Gy/hour, is equivalent to 50 Gy of external-beam radiotherapy in fractions of 2 Gy.25 Compared with conventional external-beam radiotherapy, implantation of 125I seeds involves a smaller irradiation dose, longer effect, and more accurate treatment positioning to achieve genuine image-guided radiotherapy and conformal radiotherapy. 125I seeds provide a high local dose effect to tumors, with a small radiation radius and minimal damage to surrounding normal tissue. The radiation dose from 125I seeds is inversely proportional to the square of distance, and the effective diameter of particle radiation is only 17 mm.26 Therefore, 125I seeds represent an excellent local treatment for a well-selected patient population.27

Following treatment with 125I seeds, although the patient developed redness and swelling of the skin after particle implantation, the therapeutic effect was relatively good. To prevent the occurrence of skin symptoms, hyaluronic acid injection can be used to provide space between the tumor and the skin in patients with subdermal tumors, resulting in decreased doses to the skin during brachytherapy.28

Conclusion

125I seeds represent an alternative therapeutic opportunity for patients with vulvar SCC who do not have the option of surgery and chemotherapy and for those with local relapse with lymph-node metastasis after previous radiotherapy.