Martyna Bednarczyk1, Edyta Fatyga1, Sylwia Dzięgielewska-Gęsiak1, Dariusz Waniczek2, Beniamin Grabarek3, Nikola Zmarzły3, Grażyna Janikowska4, Małgorzata Muc-Wierzgoń1. 1. Department of Internal Medicine, Medical University of Silesia in Katowice, 40-055 Katowice, Poland. 2. Department of Propedeutics Surgery, Chair of General, Colorectal and Polytrauma Surgery, Medical University of Silesia in Katowice, SHS in Katowice, 40-055 Katowice, Poland. 3. Department of Histology, Cytophysiology and Embryology, Faculty of Medicine in Zabrze, University of Technology in Katowice, 40-055 Katowice, Poland. 4. Department of Analytical Chemistry, Medical University of Silesia in Katowice, 40-055 Katowice, Poland.
Abstract
BACKGROUND: Autophagy plays a dual role of tumor suppression and tumor promotion in colorectal cancer. The study aimed to find those microRNAs (miRNAs) important in BECN1, LAMP2, and PINK1 regulation and to determine the possible role of the epigenetic changes in examined colorectal cancer using an in silico approach. METHODS: A total of 44 pairs of surgically removed tumors at clinical stages I‒IV and healthy samples (marginal tissues) from patients' guts were analyzed. Analysis of the obtained results was conducted using the PL-Grid Infrastructure and Statistica 12.0 program. The miRNAs and CpG islands were estimated using the microrna.org database and MethPrimer program. RESULTS: The autophagy-related genes were shown to be able to be regulated by miRNAs (BECN1-49 mRNA, LAMP2-62 mRNA, PINK1-6 mRNA). It was observed that promotion regions containing at least one CpG region were present in the sequence of each gene. CONCLUSIONS: The in silico analysis performed allowed us to determine the possible role of epigenetic mechanisms of regulation gene expression, which may be an interesting therapeutic target in the treatment of colorectal cancer.
BACKGROUND: Autophagy plays a dual role of tumor suppression and tumor promotion in colorectal cancer. The study aimed to find those microRNAs (miRNAs) important in BECN1, LAMP2, and PINK1 regulation and to determine the possible role of the epigenetic changes in examined colorectal cancer using an in silico approach. METHODS: A total of 44 pairs of surgically removed tumors at clinical stages I‒IV and healthy samples (marginal tissues) from patients' guts were analyzed. Analysis of the obtained results was conducted using the PL-Grid Infrastructure and Statistica 12.0 program. The miRNAs and CpG islands were estimated using the microrna.org database and MethPrimer program. RESULTS: The autophagy-related genes were shown to be able to be regulated by miRNAs (BECN1-49 mRNA, LAMP2-62 mRNA, PINK1-6 mRNA). It was observed that promotion regions containing at least one CpG region were present in the sequence of each gene. CONCLUSIONS: The in silico analysis performed allowed us to determine the possible role of epigenetic mechanisms of regulation gene expression, which may be an interesting therapeutic target in the treatment of colorectal cancer.
Authors: John J Shin; Qurratulain Aftab; Pamela Austin; Jennifer A McQueen; Tak Poon; Shu Chen Li; Barry P Young; Calvin D Roskelley; Christopher J R Loewen Journal: Dis Model Mech Date: 2016-08-12 Impact factor: 5.758