Katarzyna Haraźna1, Ewelina Cichoń2, Szymon Skibiński2, Tomasz Witko1, Daria Solarz3, Iwona Kwiecień4, Elena Marcello5, Małgorzata Zimowska1, Robert Socha1, Ewa Szefer6, Aneta Zima2, Ipsita Roy7, Konstantinos N Raftopoulos6, Krzysztof Pielichowski6, Małgorzata Witko1, Maciej Guzik1. 1. Jerzy Haber Institute of Catalysis and Surface Chemistry Polish Academy of Sciences, Niezapominajek 8, 30-239 Kraków, Poland. 2. Faculty of Materials Science and Ceramics, AGH University of Science and Technology, 30 Mickiewicza Ave., 30-059 Kraków, Poland. 3. Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, Lojasiewicza 11, 30-348 Kraków, Poland. 4. Department of Physical Chemistry and Technology of Polymers, Silesian University of Technology, M. Strzody 9, 44-100 Gliwice, Poland. 5. School of Life Sciences, College of Liberal Arts and Sciences, University of Westminster, New Cavendish Street, London W1W 6UW, UK. 6. Department of Chemistry and Technology of Polymers, Cracow University of Technology, Warszawska 24, 31-155 Kraków, Poland. 7. Department of Materials Science and Engineering, University of Sheffield, Broad Lane, Sheffield S3 7HQ, UK.
Abstract
Nowadays, regenerative medicine faces a major challenge in providing new, functional materials that will meet the characteristics desired to replenish and grow new tissue. Therefore, this study presents new ceramic-polymer composites in which the matrix consists of tricalcium phosphates covered with blends containing a chemically bounded diclofenac with the biocompatible polymer-poly(3-hydroxyoctanoate), P(3HO). Modification of P(3HO) oligomers was confirmed by NMR, IR and XPS. Moreover, obtained oligomers and their blends were subjected to an in-depth characterisation using GPC, TGA, DSC and AFM. Furthermore, we demonstrate that the hydrophobicity and surface free energy values of blends decreased with the amount of diclofenac modified oligomers. Subsequently, the designed composites were used as a substrate for growth of the pre-osteoblast cell line (MC3T3-E1). An in vitro biocompatibility study showed that the composite with the lowest concentration of the proposed drug is within the range assumed to be non-toxic (viability above 70%). Cell proliferation was visualised using the SEM method, whereas the observation of cell penetration into the scaffold was carried out by confocal microscopy. Thus, it can be an ideal new functional bone tissue substitute, allowing not only the regeneration and restoration of the defect but also inhibiting the development of chronic inflammation.
Nowadays, regenerative mepan class="Chemical">dicine faces a major challenge in providing new, functional materials that will meet the characteristics desired to replenish and grow new tissue. Therefore, this study presents new ceramic-polymer composites in which the matrix consists of tricalcium phosphates covered with blends containing a chemically bounded diclofenacwith the biocompatible polymer-poly(3-hydroxyoctanoate), P(3HO). Modification of P(3HO) oligomers was confirmed by NMR, IR and XPS. Moreover, obtained oligomers and their blends were subjected to an in-depth characterisation using GPC, TGA, DSC and AFM. Furthermore, we demonstrate that the hydrophobicity and surface free energy values of blends decreased with the amount of diclofenac modified oligomers. Subsequently, the designed composites were used as a substrate for growth of the pre-osteoblast cell line (MC3T3-E1). An in vitro biocompatibility study showed that the composite with the lowest concentration of the proposed drug is within the range assumed to be non-toxic (viability above 70%). Cell proliferation was visualised using the SEM method, whereas the observation of cell penetration into the scaffold was carried out by confocal microscopy. Thus, it can be an ideal new functional bone tissue substitute, allowing not only the regeneration and restoration of the defect but also inhibiting the development of chronic inflammation.
Entities:
Keywords:
bone regeneration; diclofenac; polyhydroxyalkanoates; polyhydroxyoctanoate oligomers; tricalcium phosphate composites
Authors: Ranjana Rai; Darmawati M Yunos; Aldo R Boccaccini; Jonathan C Knowles; Ian A Barker; Steven M Howdle; Gregory D Tredwell; Tajalli Keshavarz; Ipsita Roy Journal: Biomacromolecules Date: 2011-05-25 Impact factor: 6.988
Authors: Dina Ahmed; Hima Puthussery; Pooja Basnett; Jonathan C Knowles; Sigrun Lange; Ipsita Roy Journal: Int J Mol Sci Date: 2021-11-27 Impact factor: 5.923