Jie Wen1, Qingjing Liu2, Mengmeng Liu2, Bian Wang2, Mei Li2, Min Wang2, Xiajie Shi3, Hong Liu4, Jing Wu5. 1. Department of Endocrinology, Xiangya Hospital, Central South University, Changsha, Hunan, China; Department of Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China. 2. Department of Endocrinology, Xiangya Hospital, Central South University, Changsha, Hunan, China. 3. Department of Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China. 4. Department of Dermatology, Xiangya Hospital, Central South University, Changsha, Hunan, China. 5. Department of Endocrinology, Xiangya Hospital, Central South University, Changsha, Hunan, China. Electronic address: wujing0731@163.com.
Abstract
BACKGROUND: Chronic low-grade inflammation and dysfunction of metabolism has been reported to be involved in obesity. Regulatory T cell (Treg) and helper T cell 17 (Th17) are involved in chronic inflammatory diseases. Impaired balance of Treg/Th17 is one of the major factors contributing to inflammatory status in obesity. METHODS: Overweight/obese patients (n = 80) were recruited and classified into three subgroups: normal glucose tolerance group (NGT, n = 32), impaired glucose regulation group (IGR, n = 19) and type two diabetes mellitus group (T2DM, n = 29). Healthy individuals were paired as normal control group (NC, n = 37). We used flow cytometry to test the frequencies of circulating Treg and Th17 cells of all subjects. Serum IL-6, IL-10, TNF-α, IL-17A levels were detected by cytometric bead array and clinical information was extracted from medical records. RESULTS: In group IGR and T2DM, we revealed a severe decrease in peripheral ratio of Treg/Th17 compared with NC, but no significant difference was seen in group NGT. The serum level of IL-6 in group NGT and T2DM was higher than healthy subjects. The FPG and HbA1c levels were negatively correlated with the ratio of Treg/Th17 in overweight/obese patients. ROC curve analysis revealed that peripheral Treg/Th17 ratio <1.255 was a risk factor for prediabetes and diabetes in overweight/obese patients. CONCLUSION: Peripheral Treg/Th17 imbalance exists in overweight/obese patients with IGR or T2DM and peripheral Treg/Th17 imbalance might be a risk factor for prediabetes and diabetes in overweight/obese patients.
BACKGROUND: Chronic low-grade inflammation and dysfunction of metabolism has been reported to be involved in obesity. Regulatory T cell (Treg) and helper T cell 17 (Th17) are involved in chronic inflammatory diseases. Impaired balance of Treg/Th17 is one of the major factors contributing to inflammatory status in obesity. METHODS: Overweight/obesepatients (n = 80) were recruited and classified into three subgroups: normal glucose tolerance group (NGT, n = 32), impaired glucose regulation group (IGR, n = 19) and type two diabetes mellitus group (T2DM, n = 29). Healthy individuals were paired as normal control group (NC, n = 37). We used flow cytometry to test the frequencies of circulating Treg and Th17 cells of all subjects. Serum IL-6, IL-10, TNF-α, IL-17A levels were detected by cytometric bead array and clinical information was extracted from medical records. RESULTS: In group IGR and T2DM, we revealed a severe decrease in peripheral ratio of Treg/Th17 compared with NC, but no significant difference was seen in group NGT. The serum level of IL-6 in group NGT and T2DM was higher than healthy subjects. The FPG and HbA1c levels were negatively correlated with the ratio of Treg/Th17 in overweight/obesepatients. ROC curve analysis revealed that peripheral Treg/Th17 ratio <1.255 was a risk factor for prediabetes and diabetes in overweight/obesepatients. CONCLUSION: Peripheral Treg/Th17 imbalance exists in overweight/obesepatients with IGR or T2DM and peripheral Treg/Th17 imbalance might be a risk factor for prediabetes and diabetes in overweight/obesepatients.
Authors: Thais de Cássia Negrini; Iracilda Zeppone Carlos; Cristiane Duque; Karina Sampaio Caiaffa; Rodrigo Alex Arthur Journal: Front Oral Health Date: 2021-09-09