Melatonin mitigates UV-B stress via regulating oxidative stress response, cellular redox and alternative electron sinks in Arabidopsis thaliana.

Melatonin plays an active role in neutralizing free radicals, especially by triggering the defense system and certain enzymes that work under stress in both mammals and plant systems. Exposure to ultraviolet (UV-B) stress can be deadly for plants since UV-B can induce production of reactive oxygen species and damage nucleic acids. In the present study, to uncover the possible alleviative role of melatonin against UV-B stress, Arabidopsis thaliana plants were treated with melatonin (10 μM) and were exposed to UV-B stress for 90 min and 180 min (46 and 92 kJ m-2 d-1). Plants treated with melatonin had lower lipid peroxidation levels and higher Fv/Fm values at both time points. UV-B stress-induced activities of superoxide dismutase (SOD), glutathione reductase (GR) and ascorbate peroxidase (APX), but no additional induction was observed in melatonin treated groups. Moreover, melatonin differentially regulated the expression of glutathione peroxidase 2 (GPX2) and GPX7 genes under UV-B stress. Melatonin treatment did not have any effect on glutathione biosynthesis and catabolism genes. However, expression of alternative oxidase 1a (AOX1a) and AOX1d were lower in UV-B + melatonin treated plants when compared to only UV-B treated plants, which indicates lower oxidative load in mitochondria.