Suat Erus1, Ayşe Bilge Öztürk2, Özgür Albayrak3, Said İncir4, Murat Hüseyin Kapdağlı5, Ekin Ezgi Cesur5, Ömer Yavuz6, Serhan Tanju1, Şükrü Dilege1. 1. Department of Thoracic Surgery, Koç University School of Medicine, Istanbul, Turkey. 2. Department of Allergy and Immunology, Koç University School of Medicine, Istanbul, Turkey. 3. Koç University Research Center for Translational Medicine (KUTTAM), Istanbul, Turkey. 4. Koç University Hospital, Clinical Laboratory, Istanbul, Turkey. 5. Thoracic Surgery Department, VKF American Hospital, Istanbul, Turkey. 6. Thoracic Surgery Department, Koç University Hospital, Istanbul, Turkey.
Abstract
OBJECTIVES: Whether acute phase and immune responses are minimally affected following minimally invasive lung surgery needs further investigation. We performed a pilot study to evaluate the immune profile of patients who underwent video-assisted thoracoscopic surgery or robot-assisted thoracic surgery lobectomies for the treatment of suspicious or known stage I non-small-cell lung cancer. METHODS: Blood samples were taken preoperatively and 3 and 24 h postoperatively were analysed for C-reactive protein, glucose, cortisol, tumour necrosis factor alpha (TNF-α), interleukin 8 (IL-8) and interleukin 10 (IL-10) levels. TNF-α, IL-8 and IL-10 were also measured in lung tissues. T (CD4, CD8), B (CD19) and natural killer (CD56, CD16) cell counts and natural killer cell functions were analysed using a flow cytometry-based assay before and after surgery. RESULTS: Minimally invasive surgery (robot-assisted thoracic surgery + video-assisted thoracoscopic surgery) significantly decreased IL-10 (P = 0.016) levels after surgery. No significant differences were detected in TNF-α (P = 0.48) and IL-8 (P = 0.15) levels before and after surgery. C-reactive protein (P < 0.001), cortisol (P < 0.001) and glucose levels (P < 0.001) increased significantly after surgery. Lymphocyte, total T cell, CD3+CD4+ and CD3+CD8+ CD16+CD56+ cell counts were significantly lower on postoperative day 1. CONCLUSION: There seems to be a dynamic balance between pro- and anti-inflammatory cytokines and immune cells following minimally invasive lobectomy.
OBJECTIVES: Whether acute phase and immune responses are minimally affected following minimally invasive lung surgery needs further investigation. We performed a pilot study to evaluate the immune profile of patients who underwent video-assisted thoracoscopic surgery or robot-assisted thoracic surgery lobectomies for the treatment of suspicious or known stage I non-small-cell lung cancer. METHODS: Blood samples were taken preoperatively and 3 and 24 h postoperatively were analysed for C-reactive protein, glucose, cortisol, tumour necrosis factor alpha (TNF-α), interleukin 8 (IL-8) and interleukin 10 (IL-10) levels. TNF-α, IL-8 and IL-10 were also measured in lung tissues. T (CD4, CD8), B (CD19) and natural killer (CD56, CD16) cell counts and natural killer cell functions were analysed using a flow cytometry-based assay before and after surgery. RESULTS: Minimally invasive surgery (robot-assisted thoracic surgery + video-assisted thoracoscopic surgery) significantly decreased IL-10 (P = 0.016) levels after surgery. No significant differences were detected in TNF-α (P = 0.48) and IL-8 (P = 0.15) levels before and after surgery. C-reactive protein (P < 0.001), cortisol (P < 0.001) and glucose levels (P < 0.001) increased significantly after surgery. Lymphocyte, total T cell, CD3+CD4+ and CD3+CD8+ CD16+CD56+ cell counts were significantly lower on postoperative day 1. CONCLUSION: There seems to be a dynamic balance between pro- and anti-inflammatory cytokines and immune cells following minimally invasive lobectomy.
Authors: Mark Jaradeh; Brett Curran; Kostantinos Poulikidis; Adrian Rodrigues; Walter Jeske; Zaid M Abdelsattar; James Lubawski; Jeanine Walenga; Wickii T Vigneswaran Journal: J Thorac Dis Date: 2022-06 Impact factor: 3.005
Authors: József Furák; Tibor Németh; Judit Lantos; Csongor Fabó; Tibor Géczi; Noémi Zombori-Tóth; Dóra Paróczai; Zalán Szántó; Zsolt Szabó Journal: Front Surg Date: 2022-05-20