Harun Un1, Rustem Anil Ugan2, Muhammet Ali Gurbuz3, Yasin Bayir4, Aysenur Kahramanlar4, Gokce Kaya5, Elif Cadirci6, Zekai Halici6. 1. Agri Ibrahim Cecen University, Faculty of Pharmacy, Department of Biochemistry, Agri, Turkey. Electronic address: hun@agri.edu.tr. 2. Ataturk University, Faculty of Pharmacy, Department of Pharmacology, Erzurum, Turkey. 3. Ataturk University, Faculty of Medicine, Department of Histology and Embryology, Erzurum, Turkey. 4. Ataturk University, Faculty of Pharmacy, Department of Biochemistry, Erzurum, Turkey. 5. Ataturk University, Faculty of Medicine, Department of Pharmacology, Erzurum, Turkey. 6. Ataturk University, Faculty of Medicine, Department of Pharmacology, Erzurum, Turkey; Clinical Research, Development and Design Application and Research Center, Ataturk University, Erzurum, Turkey.
Abstract
AIM: Cisplatin (Cis) is widely used chemotherapeutic and has some serious side effects as nephrotoxicity. Phloretin (PH) and Phloridzin (PZ) are known their anti-oxidant anti-inflammatory effects. We aimed to examine the protective effects of PH and PZ on cisplatin-induced nephrotoxicity. MAIN METHODS: Totally, 48 Balb/C female mice were separated into eight groups (n = 6). First day, single dose of cisplatin (20 mg/kg intraperitoneal) was administered to induce toxicity. PH and PZ were given (50 and 100 mg/kg orally) to treatment groups during 3 days. After the experimental procedures serum renal function enzymes (BUN and Creatinine), oxidative parameters (SOD, GSH and MDA), nuclear agent NFKβ, inflammatory cytokines (Tnf-α and IL1β) and HSP70 expressions and histopathological assessments were analyzed. KEY FINDINGS: Serum enzymes, tissue cytokines and oxidative stress were increased after the Cis treatment. PH and PZ treatments normalized all parameters compared to Cis administrated group. After the treatments, SOD activities and GSH levels were increased while MDA levels were decreased. PH and PZ treatments decreased Tnf-α, IL1β and NFKβ mRNA expressions. Cis significantly increased the HSP70 expression while PH and PZ administrations significantly decreased. Similar the biochemical and molecular results, PH and PZ showed positive effects on tissue pathological parameters. Cisplatin cause a lot of abnormal structures as tubular and glomeruli damages on the kidney. SIGNIFICANCE: PH and PZ play important physiological roles in the prevention of nephrotoxicity. Antioxidant and anti-inflammatory effects of PH and PZ demonstrated visible protective effects in the cisplatin-induced nephrotoxicity model.
AIM: Cisplatin (Cis) is widely used chemotherapeutic and has some serious side effects as nephrotoxicity. Phloretin (PH) and Phloridzin (PZ) are known their anti-oxidant anti-inflammatory effects. We aimed to examine the protective effects of PH and PZ on cisplatin-induced nephrotoxicity. MAIN METHODS: Totally, 48 Balb/C female mice were separated into eight groups (n = 6). First day, single dose of cisplatin (20 mg/kg intraperitoneal) was administered to induce toxicity. PH and PZ were given (50 and 100 mg/kg orally) to treatment groups during 3 days. After the experimental procedures serum renal function enzymes (BUN and Creatinine), oxidative parameters (SOD, GSH and MDA), nuclear agent NFKβ, inflammatory cytokines (Tnf-α and IL1β) and HSP70 expressions and histopathological assessments were analyzed. KEY FINDINGS: Serum enzymes, tissue cytokines and oxidative stress were increased after the Cis treatment. PH and PZ treatments normalized all parameters compared to Cis administrated group. After the treatments, SOD activities and GSH levels were increased while MDA levels were decreased. PH and PZ treatments decreased Tnf-α, IL1β and NFKβ mRNA expressions. Cis significantly increased the HSP70 expression while PH and PZ administrations significantly decreased. Similar the biochemical and molecular results, PH and PZ showed positive effects on tissue pathological parameters. Cisplatin cause a lot of abnormal structures as tubular and glomeruli damages on the kidney. SIGNIFICANCE: PH and PZ play important physiological roles in the prevention of nephrotoxicity. Antioxidant and anti-inflammatory effects of PH and PZ demonstrated visible protective effects in the cisplatin-induced nephrotoxicity model.
Authors: Mahrous A Ibrahim; Athar M Khalifa; Alaa A Mohamed; Rania A Galhom; Horeya E Korayem; Noha M Abd El-Fadeal; Ahmed Abd-Eltawab Tammam; Mohamed Mansour Khalifa; Osama S Elserafy; Rehab I Abdel-Karim Journal: Toxics Date: 2022-09-06
Authors: Yasmina M Abd-Elhakim; Attia A A Moselhy; Adil Aldhahrani; Rasha R Beheiry; Wafaa A M Mohamed; Mohamed Mohamed Soliman; Bayan A Saffaf; Maha M El Deib Journal: Antioxidants (Basel) Date: 2021-05-21
Authors: John D Imig; Md Abdul Hye Khan; Anna Burkhan; Guan Chen; Adeniyi Michael Adebesin; John R Falck Journal: Int J Mol Sci Date: 2021-03-10 Impact factor: 5.923