Ionuț Isaia Jeican1,2, Lucian Barbu Tudoran3,4, Adrian Florea5, Mirela Flonta6, Veronica Trombitas1, Anda Apostol1, Mihai Dumitru7,8, Maria Aluaș9, Lia Monica Junie10, Silviu Albu1. 1. Department of Head and Neck Surgery and Otorhinolaryngology, University Clinical Hospital of Railway Company, Iuliu Hatieganu University of Medicine and Pharmacy, 16-20 Republicii, 400015 Cluj-Napoca, Romania. 2. Department of Anatomy and Embryology, Iuliu Hatieganu University of Medicine and Pharmacy, 3-5 Clinicilor, 400006 Cluj-Napoca, Romania. 3. Electron Microscopy Laboratory Prof. C. Craciun, Faculty of Biology and Geology, Babes-Bolyai University, 5-7 Clinicilor, 400006 Cluj-Napoca, Romania. 4. Electron Microscopy Integrated Laboratory, National Institute for R&D of Isotopic and Molecular Technologies, 67-103 Donath, 400293 Cluj-Napoca, Romania. 5. Department of Cell and Molecular Biology, Iuliu Hatieganu University of Medicine and Pharmacy, 6 Louis Pasteur, 400349 Cluj-Napoca, Romania. 6. Infectious Diseases Hospital of Cluj-Napoca, 23 Iuliu Moldovan, 400348 Cluj-Napoca, Romania. 7. Department of Head and Neck Surgery and Otorhinolaryngology, Bucharest University Emergency Hospital, 169 Splaiul Independenței, 010271 Bucharest, Romania. 8. Department of Anatomy and Embryology, Carol Davila University of Medicine and Pharmacy, 1 Frosa Sarandy, 050474 Bucharest, Romania. 9. Department of Oral Health, Iuliu Hatieganu University of Medicine and Pharmacy, 15 Victor Babes, 400012 Cluj-Napoca, Romania. 10. Department of Microbiology, Iuliu Hatieganu University of Medicine and Pharmacy, 6 Louis Pasteur Street, 400349 Cluj-Napoca, Romania.
Abstract
(1) Background: Chronic rhinosinusitis (CRS) represents a wide range of infectious-inflammatory processes affecting, simultaneously, the nose and paranasal sinuses mucosa. The paper presents outcomes of the investigation of CRS microbiological characteristics in a group of 32 patients. (2) Methods: The purulent samples were collected during functional endoscopic sinus surgery. Agar plates were incubated and examined. All types of colonies were identified using Matrix-Assisted Laser Desorption - Ionisation-Time of Flight Mass Spectrometry (MALDI-TOF MS). For scanning electron microscopy, samples were fixed and sputter-coated with 10 nm gold and analyzed using a scanning electron microscope. For transmission electron microscopy, samples were fixed, postfixed, and dehydrated. After polymerization, ultrathin sections were collected on carbon coated copper grids and analyzed with Jeol JEM1010 TEM. (3) Results: Positive microbiological diagnosis was obtained in 62.5% of cases. The most frequent species found are Staphylococcus aureus and Streptococcus constellatus subsp. pharyngis. Corynebacterium aurimucosum and Eggerthia catenaformis were unreported species in CRS until the present. Biofilm was evidenced in 43.7% of sinus mucosa samples. Ciliary disorientation, atrophy, and no ciliated cells were also identified. (4) Conclusion: The microbial factor-pathogen or opportunistic-is one of the most important pathological links in chronic rhinosinusitis. MALDI-TOF MS allows easily and quickly identification of germs.
(1) Background: Chronic rhinosinusitis (CRS) represents a wide range of infectious-inflammatory processes affecting, simultaneously, the nose and paranasal sinuses mucosa. The paper presents outcomes of the investigation of CRS microbiological characteristics in a group of 32 patients. (2) Methods: The purulent samples were collected during functional endoscopic sinus surgery. Agar plates were incubated and examined. All types of colonies were identified using Matrix-Assisted Laser Desorption - Ionisation-Time of Flight Mass Spectrometry (MALDI-TOF MS). For scanning electron microscopy, samples were fixed and sputter-coated with 10 nm gold and analyzed using a scanning electron microscope. For transmission electron microscopy, samples were fixed, postfixed, and dehydrated. After polymerization, ultrathin sections were collected on carbon coated copper grids and analyzed with Jeol JEM1010 TEM. (3) Results: Positive microbiological diagnosis was obtained in 62.5% of cases. The most frequent species found are Staphylococcus aureus and Streptococcus constellatus subsp. pharyngis. Corynebacterium aurimucosum and Eggerthia catenaformis were unreported species in CRS until the present. Biofilm was evidenced in 43.7% of sinus mucosa samples. Ciliary disorientation, atrophy, and no ciliated cells were also identified. (4) Conclusion: The microbial factor-pathogen or opportunistic-is one of the most important pathological links in chronic rhinosinusitis. MALDI-TOF MS allows easily and quickly identification of germs.
Authors: Michael Hoggard; Brett Wagner Mackenzie; Ravi Jain; Michael W Taylor; Kristi Biswas; Richard G Douglas Journal: Clin Microbiol Rev Date: 2017-01 Impact factor: 26.132
Authors: Judd H Fastenberg; Wayne D Hsueh; Ali Mustafa; Nadeem A Akbar; Waleed M Abuzeid Journal: World J Otorhinolaryngol Head Neck Surg Date: 2016-05-05