| Literature DB >> 33301089 |
Abstract
In this chapter, frequently used methods for elucidating sequence and structure of chloroplast genomes are reviewed, as a current best practice guide. This concerns methods for DNA extraction, sequencing library preparation, and bioinformatics (assembly, verification, annotation, and sequence comparisons). Recommendations for standard data reporting practices are given-chloroplast genome sequencing reports can be highly formalized, and publication in the form of standard data reports is the best option for comparison and meta-analysis purposes.Keywords: Bioinformatics; Chloroplast genome; Data reporting standards; High-throughput sequencing; Next-generation sequencing
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Year: 2021 PMID： 33301089 DOI： 10.1007/978-1-0716-0997-2_5
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745