Literature DB >> 33301087

Isolation and Purification of DNA from Complicated Biological Samples.

Ruslan Kalendar1,2, Svetlana Boronnikova3, Mervi Seppänen4.   

Abstract

The isolation of nucleic acids from a biological sample is an important step for many molecular biology applications and medical diagnostic assays. This chapter describes an efficient protocol using established acidic CTAB (with a pH value of 5.0 to 6.8) based extraction method for isolation and/or purification of high molecular weight genomic DNA from a range of fresh and difficult sources from plant, animal, fungi, and soil material. This protocol is suitable for many sequencing and genotyping applications, including large-scale sample screening.

Entities:  

Keywords:  Animal tissues; CTAB; DNA extraction; Food; Herbarium specimens; High-quality DNA; Plant tissues; Soil; Woody plants

Mesh：

Substances：

Year:  2021        PMID： 33301087     DOI： 10.1007/978-1-0716-0997-2_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  27 in total

1.  iPBS: a universal method for DNA fingerprinting and retrotransposon isolation.

Authors:  Ruslan Kalendar; Kristiina Antonius; Petr Smýkal; Alan H Schulman
Journal:  Theor Appl Genet       Date:  2010-07-10       Impact factor: 5.699

2.  Design and validation of an STR hexaplex assay for DNA profiling of grapevine cultivars.

Authors:  Jiří Drábek; Michaela Smolíková; Ruslan Kalendar; Fernando A Lopes Pinto; Pavel Pavloušek; Karel Klepárník; Ivo Frébort
Journal:  Electrophoresis       Date:  2016-10-26       Impact factor: 3.535

3.  Transposon-based tagging: IRAP, REMAP, and iPBS.

Authors:  Ruslan Kalendar; Alan H Schulman
Journal:  Methods Mol Biol       Date:  2014

4.  Alu repeats: a source for the genesis of primate microsatellites.

Authors:  S S Arcot; Z Wang; J L Weber; P L Deininger; M A Batzer
Journal:  Genomics       Date:  1995-09-01       Impact factor: 5.736

5.  Rapid isolation of high molecular weight plant DNA.

Authors:  M G Murray; W F Thompson
Journal:  Nucleic Acids Res       Date:  1980-10-10       Impact factor: 16.971

6.  A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide.

Authors:  G C Allen; M A Flores-Vergara; S Krasynanski; S Kumar; W F Thompson
Journal:  Nat Protoc       Date:  2006       Impact factor: 13.491

7.  High-throughput retrotransposon-based genetic diversity of maize germplasm assessment and analysis.

Authors:  Marwa Ghonaim; Ruslan Kalendar; Hoda Barakat; Nahla Elsherif; Naglaa Ashry; Alan H Schulman
Journal:  Mol Biol Rep       Date:  2020-01-09       Impact factor: 2.316

8.  Fingerprinting genomes using PCR with arbitrary primers.

Authors:  J Welsh; M McClelland
Journal:  Nucleic Acids Res       Date:  1990-12-25       Impact factor: 16.971

9.  An efficient method for genomic DNA extraction from different molluscs species.

Authors:  Jorge C Pereira; Raquel Chaves; Estela Bastos; Alexandra Leitão; Henrique Guedes-Pinto
Journal:  Int J Mol Sci       Date:  2011-11-17       Impact factor: 5.923

10.  Palindromic sequence-targeted (PST) PCR: a rapid and efficient method for high-throughput gene characterization and genome walking.

Authors:  Ruslan Kalendar; Alexandr V Shustov; Mervi M Seppänen; Alan H Schulman; Frederick L Stoddard
Journal:  Sci Rep       Date:  2019-11-27       Impact factor: 4.379
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  7 in total

1.  Isolation and identification of marine Bacillus altitudinis KB1 from coastal Kerala: asparaginase producer.

Authors:  Wael Ali Mohammed Hadi; Boby T Edwin; Ananthakrishnan Jayakumaran Nair
Journal:  J Mar Bio Assoc India       Date:  2021-10-30

2.  Primer Binding Site (PBS) Profiling of Genetic Diversity of Natural Populations of Endemic Species Allium ledebourianum Schult.

Authors:  Oxana Khapilina; Ainur Turzhanova; Alevtina Danilova; Asem Tumenbayeva; Vladislav Shevtsov; Yuri Kotukhov; Ruslan Kalendar
Journal:  BioTech (Basel)       Date:  2021-10-13

3.  DNA profiling and assessment of genetic diversity of relict species Allium altaicum Pall. on the territory of Altai.

Authors:  Oxana Khapilina; Olesya Raiser; Alevtina Danilova; Vladislav Shevtsov; Ainur Turzhanova; Ruslan Kalendar
Journal:  PeerJ       Date:  2021-01-08       Impact factor: 2.984

4.  Modified "Allele-Specific qPCR" Method for SNP Genotyping Based on FRET.

Authors:  Ruslan Kalendar; Akmaral Baidyussen; Dauren Serikbay; Lyudmila Zotova; Gulmira Khassanova; Marzhan Kuzbakova; Satyvaldy Jatayev; Yin-Gang Hu; Carly Schramm; Peter A Anderson; Colin L D Jenkins; Kathleen L Soole; Yuri Shavrukov
Journal:  Front Plant Sci       Date:  2022-01-10       Impact factor: 5.753

Review 5.  A Review of Isothermal Amplification Methods and Food-Origin Inhibitors against Detecting Food-Borne Pathogens.

Authors:  Ye-Ji Moon; So-Young Lee; Se-Wook Oh
Journal:  Foods       Date:  2022-01-24

6.  Screening a collection of local and foreign varieties of Solanum lycopersicum L. in Kazakhstan for genetic markers of resistance against three tomato viruses.

Authors:  Alexandr Pozharskiy; Valeriya Kostyukova; Aisha Taskuzhina; Gulnaz Nizamdinova; Nina Kisselyova; Ruslan Kalendar; Nurlybek Karimov; Dilyara Gritsenko
Journal:  Heliyon       Date:  2022-08-10

7.  Genetic Diversity and Pathogenicity of Rhizoctonia spp. Isolates Associated with Red Cabbage in Samsun (Turkey).

Authors:  Ismail Erper; Goksel Ozer; Ruslan Kalendar; Sirin Avci; Elif Yildirim; Mehtap Alkan; Muharrem Turkkan
Journal:  J Fungi (Basel)       Date:  2021-03-21
  7 in total

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