Lidiya Vysotskaya1, Guzel Akhiyarova1, Arina Feoktistova1, Zarina Akhtyamova1, Alla Korobova1, Igor Ivanov1, Ian Dodd2, Bulat Kuluev3,4, Guzel Kudoyarova1,4. 1. Ufa Institute of Biology of Ufa Federal Research Centre of the Russian Academy of Sciences, Pr. Octyabrya, 69, 450054 Ufa, Russia. 2. The Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, UK. 3. Institute of Biochemistry and Genetics of Ufa Federal Research Centre of Russian Academy of Sciences, Pr. Octyabrya, 71, 450054 Ufa, Russia. 4. Department of Biology, Bashkir State University, Zaki-Validi St. 32, 450074 Ufa, Russia.
Abstract
Although changes in root architecture in response to the environment can optimize mineral and water nutrient uptake, mechanisms regulating these changes are not well-understood. We investigated whether P deprivation effects on root development are mediated by abscisic acid (ABA) and its interactions with other hormones. The ABA-deficient barley mutant Az34 and its wild-type (WT) were grown in P-deprived and P-replete conditions, and hormones were measured in whole roots and root tips. Although P deprivation decreased growth in shoot mass similarly in both genotypes, only the WT increased primary root length and number of lateral roots. The effect was accompanied by ABA accumulation in root tips, a response not seen in Az34. Increased ABA in P-deprived WT was accompanied by decreased concentrations of cytokinin, an inhibitor of root extension. Furthermore, P-deficiency in the WT increased auxin concentration in whole root systems in association with increased root branching. In the ABA-deficient mutant, P-starvation failed to stimulate root elongation or promote branching, and there was no decline in cytokinin and no increase in auxin. The results demonstrate ABA's ability to mediate in root growth responses to P starvation in barley, an effect linked to its effects on cytokinin and auxin concentrations.
Although changes in root architecture in response to the environment can opn>timize mineral and n>an class="Chemical">water nutrient uptake, mechanisms regulating these changes are not well-understood. We investigated whether P deprivation effects on root development are mediated by abscisic acid (ABA) and its interactions with other hormones. The ABA-deficient barley mutant Az34 and its wild-type (WT) were grown in P-deprived and P-replete conditions, and hormones were measured in whole roots and root tips. Although P deprivation decreased growth in shoot mass similarly in both genotypes, only the WT increased primary root length and number of lateral roots. The effect was accompanied by ABA accumulation in root tips, a response not seen in Az34. Increased ABA in P-deprived WT was accompanied by decreased concentrations of cytokinin, an inhibitor of root extension. Furthermore, P-deficiency in the WT increased auxin concentration in whole root systems in association with increased root branching. In the ABA-deficient mutant, P-starvation failed to stimulate root elongation or promote branching, and there was no decline in cytokinin and no increase in auxin. The results demonstrate ABA's ability to mediate in root growth responses to P starvation in barley, an effect linked to its effects on cytokinin and auxin concentrations.
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