| Literature DB >> 33289891 |
Martin C Frith1,2,3, Satomi Mitsuhashi4,5, Kazutaka Katoh6,7.
Abstract
Long DNA and RNA reads from nanopore and PacBio technologies have many applications, but the raw reads have a substantial error rate. More accurate sequences can be obtained by merging multiple reads from overlapping parts of the same sequence. lamassemble aligns up to ∼1000 reads to each other, and makes a consensus sequence, which is often much more accurate than the raw reads. It is useful for studying a region of interest such as an expanded tandem repeat or other disease-causing mutation.Keywords: Dotplot; Genomic variations; LAST; MAFFT; Nanopore; PacBio; Paralogy; Repeat expansion disease; Strand-asymmetric error pattern; last-train
Mesh:
Year: 2021 PMID: 33289891 DOI: 10.1007/978-1-0716-1036-7_9
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745