BACKGROUND: Carbon dots (C-dots) with antimicrobial activity were synthesized from the white mulberry extract with the aim of fabricating anti-listeria nanopaper using bacterial nanocellulose (BNC). Highly dispersed synthesized C-dots with a size smaller than 10 nm (approximately 4.9 nm) were impregnated into BNC by an ex situ coating method and then mechanical, morphological, UV-protectant and antibacterial activity were assessed. Randomized response surface methodology using a central composite design was applied to investigate the optimized concentration of C-dots in the BNC membrane. RESULTS: An optimized nanopaper including C-dots at a concentration of 530 g L-1 and an impregnation time of 14 h at 30 °C with significant antimicrobial activity on Listeria monocytogenes was designed. The addition of C-dots into BNC significantly increased ultimate tensile strength and decreased strain with respect to breaking BNC. A BNC sheet with high-efficient UV-blocking property was prepared using C-dots. CONCLUSION: Based on the results, the designed nanopaper shows a substantial capacity with respect to the fabrication of antimicrobial/UV-blocking sheets for food active packaging.
BACKGROUND:Carbon dots (C-dots) with antimicrobial activity were synthesized from the white mulberry extract with the aim of fabricating anti-listeria nanopaper using bacterial nanocellulose (BNC). Highly dispersed synthesized C-dots with a size smaller than 10 nm (approximately 4.9 nm) were impregnated into BNC by an ex situ coating method and then mechanical, morphological, UV-protectant and antibacterial activity were assessed. Randomized response surface methodology using a central composite design was applied to investigate the optimized concentration of C-dots in the BNC membrane. RESULTS: An optimized nanopaper including C-dots at a concentration of 530 g L-1 and an impregnation time of 14 h at 30 °C with significant antimicrobial activity on Listeria monocytogenes was designed. The addition of C-dots into BNC significantly increased ultimate tensile strength and decreased strain with respect to breaking BNC. A BNC sheet with high-efficient UV-blocking property was prepared using C-dots. CONCLUSION: Based on the results, the designed nanopaper shows a substantial capacity with respect to the fabrication of antimicrobial/UV-blocking sheets for food active packaging.