Eva Maria Parisio1, Giulio Camarlinghi1, Marco Coppi2, Claudia Niccolai3, Alberto Antonelli4, Maria Nardone1, Chiara Vettori1, Tommaso Giani4, Romano Mattei1, Gian Maria Rossolini4. 1. Clinical Chemistry and Microbiology Analysis Unit, San Luca Hospital, USL Toscana Nord Ovest, Lucca, Italy. 2. Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy; Microbiology and Virology Unit, Florence Careggi University Hospital, Florence, Italy. Electronic address: marco.coppi@unifi.it. 3. Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy. 4. Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy; Microbiology and Virology Unit, Florence Careggi University Hospital, Florence, Italy.
Abstract
OBJECTIVES: To compare fosfomycin susceptibility testing with the commercial agar dilution (AD) test, AD fosfomycin (Liofilchem, Roseto degli Abruzzi, Italy) and the reference AD method, using a collection of multidrug-resistant (MDR) Enterobacterales and Pseudomonas aeruginosa clinical isolates. METHODS: The collection included 119 carbapenemase-producing Enterobacterales, 53 Enterobacterales producing acquired AmpC-type and/or extended-spectrum β-lactamases, and 38 carbapenemase-producing P. aeruginosa, including representatives of different high-risk clones. AD Fosfomycin and AD reference method (ISO 20776-1:2019) were performed starting from the same microbial suspension. Results were interpreted according to EUCAST clinical breakpoints (10.0). Essential agreement (EA), category agreement (CA) and error rates were calculated as described by the International Organization for Standardization. RESULTS: Of 172 Enterobacterales, 143 (83.1%, including 92.9% (52 of 56) of the NDM-producers and 84.2% (48 of 57) of the KPC-producers) were susceptible to fosfomycin using reference AD. A CA of 91.9% (158 of 172; 95% confidence interval (CI):87.1-95.3) and an EA of 92.5% (136 of 147; CI:87.4-96.0), respectively, were calculated for the commercial AD Fosfomycin test, with 9.8% (14 of 128) of major errors and no very major errors (0 of 29). Overall, 86.8% (33 of 38) of P. aeruginosa showed a fosfomycin MIC ≤128 mg/L using reference AD. An EA of 84.8% (CI:66.3-92.0) was calculated for the commercial AD Fosfomycin test, with a CA of 100% (CI:93.6-100) when considering a tentative breakpoint at 128 mg/L. CONCLUSIONS: AD Fosfomycin showed an overall good concordance compared to reference AD.
OBJECTIVES: To compare fosfomycin susceptibility testing with the commercial agar dilution (AD) test, AD fosfomycin (Liofilchem, Roseto degli Abruzzi, Italy) and the reference AD method, using a collection of multidrug-resistant (MDR) Enterobacterales and Pseudomonas aeruginosa clinical isolates. METHODS: The collection included 119 carbapenemase-producing Enterobacterales, 53 Enterobacterales producing acquired AmpC-type and/or extended-spectrum β-lactamases, and 38 carbapenemase-producing P. aeruginosa, including representatives of different high-risk clones. AD Fosfomycin and AD reference method (ISO 20776-1:2019) were performed starting from the same microbial suspension. Results were interpreted according to EUCAST clinical breakpoints (10.0). Essential agreement (EA), category agreement (CA) and error rates were calculated as described by the International Organization for Standardization. RESULTS: Of 172 Enterobacterales, 143 (83.1%, including 92.9% (52 of 56) of the NDM-producers and 84.2% (48 of 57) of the KPC-producers) were susceptible to fosfomycin using reference AD. A CA of 91.9% (158 of 172; 95% confidence interval (CI):87.1-95.3) and an EA of 92.5% (136 of 147; CI:87.4-96.0), respectively, were calculated for the commercial AD Fosfomycin test, with 9.8% (14 of 128) of major errors and no very major errors (0 of 29). Overall, 86.8% (33 of 38) of P. aeruginosa showed a fosfomycin MIC ≤128 mg/L using reference AD. An EA of 84.8% (CI:66.3-92.0) was calculated for the commercial AD Fosfomycin test, with a CA of 100% (CI:93.6-100) when considering a tentative breakpoint at 128 mg/L. CONCLUSIONS: AD Fosfomycin showed an overall good concordance compared to reference AD.