Suneeta Chimalapati1,2, Alexander E Lafrance1, Luming Chen1, Kim Orth1,2,3. 1. Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas. 2. Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas. 3. Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas.
Abstract
Vibrio parahaemolyticus is a Gram-negative, halophilic bacterium and opportunistic pathogen of humans and shrimp. Investigating the mechanisms of V. parahaemolyticus infection and the multifarious virulence factors it employs requires procedures for bacterial culture, genetic manipulation, and analysis of virulence phenotypes. Detailed protocols for growth assessment, generation of mutants, and phenotype assessment are included in this article.
Vibrio parahaemolyticus is a Gram-negative, halophilic bacterium and opportunistic pathogen of humans and shrimp. Investigating the mechanisms of V. parahaemolyticus infection and the multifarious virulence factors it employs requires procedures for bacterial culture, genetic manipulation, and analysis of virulence phenotypes. Detailed protocols for growth assessment, generation of mutants, and phenotype assessment are included in this article.
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