Qingling Liu1, Chengbin Lei2. 1. Department of Clinical Laboratory, Zibo Maternal and Child Health Hospital, No. 11 Xing Yuan Dong Road, Zibo 255000, China. 2. Department of Clinical Laboratory, Zibo Central Hospital, Gongqingtuan West Road, Zibo 255036, China. Electronic address: chengbin_ad@163.com.
Abstract
BACKGROUND: Alzheimer's disease (AD) is a common neurodegenerative disease with an increasing incidence rate. Numerous microRNAs (miRNAs) have been found to be involved in AD progression. This study aimed to investigate the expression and diagnostic value of microRNA-331-3p (miR-331-3p) in AD patients and to explore the effects of miR-331-3p on neuronal viability and neuroinflammation. METHODS: This study recruited AD patients and used Aβ1-40 treated SH-SY5Y cells mimicking AD characteristics. The expression of miR-331-3p was estimated using reverse transcription quantitative PCR. A receiver operating characteristic (ROC) analysis was used to evaluate the diagnostic value of miR-331-3p, and the correlation of miR-331-3p with patients' Mini-Mental State Examination (MMSE) scores and serum proinflammatory cytokines were analyzed. The effects of miR-331-3p on neuronal viability and inflammatory response were explored in SH-SY5Y cells by in vitro analysis. RESULTS: In AD patients and Aβ1-40 treated SH-SY5Y cells, the expression of miR-331-3p was significantly downregulated. Serum miR-331-3p had certain diagnostic potential and was correlated with the MMSE scores and serum proinflammatory cytokine levels of AD patients. In Aβ1-40-treated SH-SY5Y cells, the overexpression of miR-331-3p enhanced cell viability and inhibited inflammatory responses. CONCLUSION: The data of this study indicated that serum expression of miR-331-3p is decreased in AD patients, and is correlated with the MMSE scores and proinflammatory cytokine levels of AD patients. In addition, miR-331-3p can regulate the cell viability and the expression of pro-inflammatory cytokines of Aβ1-40 treated SH-SY5Y cells, indicating the potential neuroprotective role of miR-331-3p.
BACKGROUND:Alzheimer's disease (AD) is a common neurodegenerative disease with an increasing incidence rate. Numerous microRNAs (miRNAs) have been found to be involved in AD progression. This study aimed to investigate the expression and diagnostic value of microRNA-331-3p (miR-331-3p) in ADpatients and to explore the effects of miR-331-3p on neuronal viability and neuroinflammation. METHODS: This study recruited ADpatients and used Aβ1-40 treated SH-SY5Y cells mimicking AD characteristics. The expression of miR-331-3p was estimated using reverse transcription quantitative PCR. A receiver operating characteristic (ROC) analysis was used to evaluate the diagnostic value of miR-331-3p, and the correlation of miR-331-3p with patients' Mini-Mental State Examination (MMSE) scores and serum proinflammatory cytokines were analyzed. The effects of miR-331-3p on neuronal viability and inflammatory response were explored in SH-SY5Y cells by in vitro analysis. RESULTS: In ADpatients and Aβ1-40 treated SH-SY5Y cells, the expression of miR-331-3p was significantly downregulated. Serum miR-331-3p had certain diagnostic potential and was correlated with the MMSE scores and serum proinflammatory cytokine levels of ADpatients. In Aβ1-40-treated SH-SY5Y cells, the overexpression of miR-331-3p enhanced cell viability and inhibited inflammatory responses. CONCLUSION: The data of this study indicated that serum expression of miR-331-3p is decreased in ADpatients, and is correlated with the MMSE scores and proinflammatory cytokine levels of ADpatients. In addition, miR-331-3p can regulate the cell viability and the expression of pro-inflammatory cytokines of Aβ1-40 treated SH-SY5Y cells, indicating the potential neuroprotective role of miR-331-3p.
Authors: Theresa A Lusardi; Ursula S Sandau; Nikita A Sakhanenko; Sarah Catherine B Baker; Jack T Wiedrick; Jodi A Lapidus; Murray A Raskind; Ge Li; Elaine R Peskind; David J Galas; Joseph F Quinn; Julie A Saugstad Journal: Front Neurosci Date: 2021-09-09 Impact factor: 4.677