Denny Sakkas1, Colin M Howles2, Leslie Atkinson3, Andrea Borini4, Ernesto A Bosch3, Crystal Bryce5, Monica Cattoli4, Alan B Copperman6, Astrid Finet de Bantel7, Brian French8, Jan Gerris9, Steve W Granger5, Veronika Grzegorczyk-Martin7, Joseph A Lee6, Michael J Levy10, Marla J Matin5, Sara Somers9, Eric A Widra10, Michael M Alper8. 1. Boston IVF, 130 Second Ave, Waltham MA 02451, USA. Electronic address: dsakkas@bostonivf.com. 2. ARIES Consulting, Ch du Barbolet 5b, 1213 Onex, Geneva, Switzerland. 3. IVI Valencia, Placa de la Policia Local, 3, 46015 Valencia, Spain. 4. Tecnobios Procreazione S.r.l Via Dante 15-40125 Bologna, Italy. 5. Salimetrics LLC, 5962 La Place Court Suite 275, CA 92008, USA. 6. Reproductive Medicine Associates of New York, 635 Madison Ave 10th floor, New York, NY 10022, USA. 7. Clinique MATHILDE, Laboratoire d'Assistance Médicale à la Procréation, 7, Boulevard de l'Europe, 76100 Rouen, France. 8. Boston IVF, 130 Second Ave, Waltham MA 02451, USA. 9. Ghent University Hospital, Vrouwenkliniek C. Heymanslaan 10, 9000 Ghent, Belgium. 10. Shady Grove Fertility, 9601 Blackwell Rd 4th floor, Rockville, MD 20850, USA.
Abstract
RESEARCH QUESTION: Ovarian stimulation during IVF cycles involves close monitoring of oestradiol, progesterone and ultrasound measurements of follicle growth. In contrast to blood draws, sampling saliva is less invasive. Here, a blind validation is presented of a novel saliva-based oestradiol and progesterone assay carried out in samples collected in independent IVF clinics. DESIGN: Concurrent serum and saliva samples were collected from 324 patients at six large independent IVF laboratories. Saliva samples were frozen and run blinded. A further 18 patients had samples collected more frequently around the time of HCG trigger. Saliva samples were analysed using an immunoassay developed with Salimetrics LLC. RESULTS: In total, 652 pairs of saliva and serum oestradiol were evaluated, with correlation coefficients ranging from 0.68 to 0.91. In the European clinics, a further 237 of saliva and serum progesterone samples were evaluated; however, the correlations were generally poorer, ranging from -0.02 to 0.22. In the patients collected more frequently, five out of 18 patients (27.8%) showed an immediate decrease in oestradiol after trigger. When progesterone samples were assessed after trigger, eight out of 18 (44.4%) showed a continued rise. CONCLUSIONS: Salivary oestradiol hormone testing correlates well to serum-based assessment, whereas progesterone values, around the time of trigger, are not consistent from patient to patient.
RESEARCH QUESTION: Ovarian stimulation during IVF cycles involves close monitoring of oestradiol, progesterone and ultrasound measurements of follicle growth. In contrast to blood draws, sampling saliva is less invasive. Here, a blind validation is presented of a novel saliva-based oestradiol and progesterone assay carried out in samples collected in independent IVF clinics. DESIGN: Concurrent serum and saliva samples were collected from 324 patients at six large independent IVF laboratories. Saliva samples were frozen and run blinded. A further 18 patients had samples collected more frequently around the time of HCG trigger. Saliva samples were analysed using an immunoassay developed with Salimetrics LLC. RESULTS: In total, 652 pairs of saliva and serum oestradiol were evaluated, with correlation coefficients ranging from 0.68 to 0.91. In the European clinics, a further 237 of saliva and serum progesterone samples were evaluated; however, the correlations were generally poorer, ranging from -0.02 to 0.22. In the patients collected more frequently, five out of 18 patients (27.8%) showed an immediate decrease in oestradiol after trigger. When progesterone samples were assessed after trigger, eight out of 18 (44.4%) showed a continued rise. CONCLUSIONS: Salivary oestradiol hormone testing correlates well to serum-based assessment, whereas progesterone values, around the time of trigger, are not consistent from patient to patient.
Authors: Roger J Hart; Thomas D'Hooghe; Eline A F Dancet; Ramón Aurell; Bruno Lunenfeld; Raoul Orvieto; Antonio Pellicer; Nikolaos P Polyzos; Wenjing Zheng Journal: Reprod Sci Date: 2021-11-15 Impact factor: 2.924