Literature DB >> 3327913

A rapid and efficient method for plasmid transformation of Klebsiella pneumoniae and Escherichia coli.

M J Merrick1, J R Gibbins, J R Postgate.   

Abstract

A rapid and efficient method for plasmid transformation of Klebsiella pneumoniae M5a1 and Escherichia coli K12 has been developed. The method, which uses a freeze-thaw cycle in the presence of CaCl2 to facilitate DNA uptake, is substantially more efficient for K. pneumoniae M5a1 than the conventional transformation procedure for E. coli. The simplicity and speed of the method makes it very attractive for routine transformation of K. pneumoniae M5a1 and E. coli K12.

Entities:  

Mesh：

Year:  1987        PMID： 3327913     DOI： 10.1099/00221287-133-8-2053

Source DB:  PubMed          Journal:  J Gen Microbiol        ISSN： 0022-1287


  14 in total

1.  Identification of clinical isolates of indole-positive Klebsiella spp., including Klebsiella planticola, and a genetic and molecular analysis of their beta-lactamases.

Authors:  Y Liu; B J Mee; L Mulgrave
Journal:  J Clin Microbiol       Date:  1997-09       Impact factor: 5.948

2.  Colicin E1 export in Salmonella typhimurium wild-type and lipopolysaccharide mutants.

Authors:  B M Viejo; M Regué; S Camprubí; J M Tomás
Journal:  Folia Microbiol (Praha)       Date:  1991       Impact factor: 2.099

3.  Lysis of Escherichia coli by the bacteriophage phi X174 E protein: inhibition of lysis by heat shock proteins.

Authors:  K D Young; R J Anderson; R J Hafner
Journal:  J Bacteriol       Date:  1989-08       Impact factor: 3.490

4.  Development of rapid microwave-mediated and low-temperature bacterial transformations.

Authors:  Valerie T Tripp; Johnathan C Maza; Douglas D Young
Journal:  J Chem Biol       Date:  2013-05-21

5.  Characterization of the membrane quinoprotein glucose dehydrogenase from Escherichia coli and characterization of a site-directed mutant in which histidine-262 has been changed to tyrosine.

Authors:  G E Cozier; R A Salleh; C Anthony
Journal:  Biochem J       Date:  1999-06-15       Impact factor: 3.857

6.  Suppression of β-Lactam Resistance by Aspergillomarasmine A Is Influenced by both the Metallo-β-Lactamase Target and the Antibiotic Partner.

Authors:  Caitlyn M Rotondo; David Sychantha; Kalinka Koteva; Gerard D Wright
Journal:  Antimicrob Agents Chemother       Date:  2020-03-24       Impact factor: 5.191

7.  Structure-function relationships in the alpha subunit of Klebsiella pneumoniae nitrogenase MoFe protein from analysis of nifD mutants.

Authors:  D Govezensky; A Zamir
Journal:  J Bacteriol       Date:  1989-10       Impact factor: 3.490

8.  Controlled expression in Klebsiella pneumoniae and Shigella flexneri using a bacteriophage P1-derived C1-regulated promoter system.

Authors:  D A Schofield; C Westwater; J W Dolan; M G Schmidt; J S Norris
Journal:  J Bacteriol       Date:  2001-12       Impact factor: 3.490

9.  Nucleotide sequence and genetic analysis of the Azotobacter chroococcum nifUSVWZM gene cluster, including a new gene (nifP) which encodes a serine acetyltransferase.

Authors:  D J Evans; R Jones; P R Woodley; J R Wilborn; R L Robson
Journal:  J Bacteriol       Date:  1991-09       Impact factor: 3.490

Review 10.  Abiotic Gene Transfer: Rare or Rampant?

Authors:  Tadej Kotnik; James C Weaver
Journal:  J Membr Biol       Date:  2016-04-11       Impact factor: 1.843
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.