| Literature DB >> 33278791 |
F Xavier López-Labrador1, Julianne R Brown2, Nicole Fischer3, Heli Harvala4, Sander Van Boheemen5, Ondrej Cinek6, Arzu Sayiner7, Tina Vasehus Madsen8, Eeva Auvinen9, Verena Kufner10, Michael Huber11, Christophe Rodriguez12, Marcel Jonges13, Mario Hönemann14, Petri Susi15, Hugo Sousa16, Paul E Klapper17, Alba Pérez-Cataluňa18, Marta Hernandez19, Richard Molenkamp20, Lia van der Hoek21, Rob Schuurman22, Natacha Couto23, Karoline Leuzinger24, Peter Simmonds25, Martin Beer26, Dirk Höper27, Sergio Kamminga28, Mariet C W Feltkamp29, Jesús Rodríguez-Díaz30, Els Keyaerts31, Xiaohui Chen Nielsen32, Elisabeth Puchhammer-Stöckl33, Aloys C M Kroes34, Javier Buesa35, Judy Breuer36, Eric C J Claas37, Jutte J C de Vries38.
Abstract
Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into clinical application. To support the development, implementation and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mHTS for viral diagnostics to share methodologies and experiences, and to develop application recommendations. This manuscript aims to provide practical recommendations for the wet lab procedures necessary for implementation of mHTS for virus diagnostics and to give recommendations for development and validation of laboratory methods, including mHTS quality assurance, control and quality assessment protocols.Entities:
Keywords: High-throughput sequencing; Next-generation sequencing; Recommendations; Viral metagenomics; Wet lab
Year: 2020 PMID： 33278791 DOI： 10.1016/j.jcv.2020.104691
Source DB: PubMed Journal: J Clin Virol ISSN： 1386-6532 Impact factor: 3.168