Ting-Tiao Pan1,2, Wang Guo1, Ping Lu1, Deyu Hu1. 1. State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for R&D of Fine Chemicals of Guizhou University, Guiyang, China. 2. College of Biological Sciences and Agriculture, Qiannan Normal University for Nationalities, Duyun, China.
Abstract
BACKGROUND: Pesticide residues in agricultural products and foods pose a serious threat to human health, and therefore a simple, rapid and direct method is urgently needed for pesticide residue detection. In addition to realizing the detection of acetamiprid in cabbage extract solution, the main target of this study was to establish an in situ surface-enhanced Raman scattering (SERS) method, which could directly detect acetamiprid residue on cabbage leaf without the need for extraction. Acetamiprid was first used to contaminate the surface of fresh cabbage leaf, and then bimetallic silver-coated gold nanoparticles (Au@AgNPs) were added on the contaminated spots and dried for SERS measurement. RESULTS: Results suggested that acetamiprid can be detected in cabbage extract and on cabbage leaf surface in situ using the SERS method based on the Au@AgNPs substrate. The limit of detection was 0.08 μg mL-1 in cabbage extract and 0.14 mg kg-1 on cabbage leaf, the recovery ranged from 80.5% to 105.5% and the relative standard deviation was in the range 4.37-10.63%. CONCLUSIONS: The proposed SERS method provides an in situ, nondestructive and rapid way to detect acetamiprid residue on the surface of fruits and vegetables, which could serve as an auxiliary approach for early screening of contaminated produce in field or on site in the future.
BACKGROUND: Pesticide residues in agricultural products and foods pose a serious threat to human health, and therefore a simple, rapid and direct method is urgently needed for pesticide residue detection. In addition to realizing the detection of acetamiprid in cabbage extract solution, the main target of this study was to establish an in situ surface-enhanced Raman scattering (SERS) method, which could directly detect acetamiprid residue on cabbage leaf without the need for extraction. Acetamiprid was first used to contaminate the surface of fresh cabbage leaf, and then bimetallic silver-coated gold nanoparticles (Au@AgNPs) were added on the contaminated spots and dried for SERS measurement. RESULTS: Results suggested that acetamiprid can be detected in cabbage extract and on cabbage leaf surface in situ using the SERS method based on the Au@AgNPs substrate. The limit of detection was 0.08 μg mL-1 in cabbage extract and 0.14 mg kg-1 on cabbage leaf, the recovery ranged from 80.5% to 105.5% and the relative standard deviation was in the range 4.37-10.63%. CONCLUSIONS: The proposed SERS method provides an in situ, nondestructive and rapid way to detect acetamiprid residue on the surface of fruits and vegetables, which could serve as an auxiliary approach for early screening of contaminated produce in field or on site in the future.