Christudas Sunil1, Santiagu Stephen Irudayaraj2, Veeramuthu Duraipandiyan3, Sara T Alrashood4, Sulaiman Ali Alharbi5, Savarimuthu Ignacimuthu6. 1. Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai, 600 034, India; Food Science and Technology Programme, Beijing Normal University-Hong Kong Baptist University United International College, Zhuhai, Guangdong, 519087, China. Electronic address: sunilcology@yahoo.co.in. 2. Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai, 600 034, India; St. Xavier's College, Maharo, Dumka, 814101, Jharkhand, India. 3. Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai, 600 034, India. Electronic address: avdpandiyan@yahoo.co.in. 4. Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, 11451, Saudi Arabia. 5. Department of Botany and Microbiology, College of Science, King Saudi University, P.O Box 2455, Riyadh, 11451, Saudi Arabia. 6. Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai, 600 034, India.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Demand for plant-based medications and therapeutics is increasing worldwide as of its potential effects and no toxic. Traditionally, so many medicinal plants are used to treat diabetes. Subsequently, investigation on medicinal plants was enduring to discover potential antidiabetic drugs. A. tetracantha is used traditionally to cure diabetes mellitus, cough, dropsy, chronic diarrhea, rheumatism, phthisis and smallpox. Scientifically, A. tetracantha has been reported as an antidiabetic agent. Friedelin, the isolated compound has been reported as hypolipidemic, antioxidant, scavenging of free radicals, antiulcer, anti-inflammatory, analgesic and antipyretic agent. AIM OF THE STUDY: To scrutinize the mechanism of antidiabetic activity of friedelin isolated from the leaves of A. tetracantha. MATERIAL AND METHODS: A. tetracantha leaves powder (5 kg) was soaked in hexane (15 liters) to obtain hexane extract. Using column chromatography, the hexane extract was fractionated using the combination of solvents like hexane and ethyl acetate. 25 fractions were obtained and the fraction 13 and 14 yielded the compound, friedelin. Friedelin at the doses of 20 and 40 mg/kg were used to treated STZ -induced diabetic rats for 28 days. Later 28 days of treatment, the bodyweight changes, levels of blood glucose, insulin, SGOT, SGPT, SALP, liver glycogen and total protein were assessed. RESULTS: Friedelin significantly brought these altered levels to near normal. Moreover, friedelin also enhanced the translocation as well as activation of GLUT2 and GLUT4 through PI3K/p-Akt signaling cascade in skeletal muscles and liver on diabetic rats. CONCLUSION: This finding proved that friedelin has an anti-diabetic effect through insulin-dependent signaling cascade mechanism, thus it may lead to establish a drug to treat type 2 diabetes mellitus.
ETHNOPHARMACOLOGICAL RELEVANCE: Demand for plant-based medications and therapeutics is increasing worldwide as of its potential effects and no toxic. Traditionally, so many medicinal plants are used to treat diabetes. Subsequently, investigation on medicinal plants was enduring to discover potential antidiabetic drugs. A. tetracantha is used traditionally to cure diabetes mellitus, cough, dropsy, chronic diarrhea, rheumatism, phthisis and smallpox. Scientifically, A. tetracantha has been reported as an antidiabetic agent. Friedelin, the isolated compound has been reported as hypolipidemic, antioxidant, scavenging of free radicals, antiulcer, anti-inflammatory, analgesic and antipyretic agent. AIM OF THE STUDY: To scrutinize the mechanism of antidiabetic activity of friedelin isolated from the leaves of A. tetracantha. MATERIAL AND METHODS: A. tetracantha leaves powder (5 kg) was soaked in hexane (15 liters) to obtain hexane extract. Using column chromatography, the hexane extract was fractionated using the combination of solvents like hexane and ethyl acetate. 25 fractions were obtained and the fraction 13 and 14 yielded the compound, friedelin. Friedelin at the doses of 20 and 40 mg/kg were used to treated STZ -induced diabeticrats for 28 days. Later 28 days of treatment, the bodyweight changes, levels of blood glucose, insulin, SGOT, SGPT, SALP, liver glycogen and total protein were assessed. RESULTS:Friedelin significantly brought these altered levels to near normal. Moreover, friedelin also enhanced the translocation as well as activation of GLUT2 and GLUT4 through PI3K/p-Akt signaling cascade in skeletal muscles and liver on diabeticrats. CONCLUSION: This finding proved that friedelin has an anti-diabetic effect through insulin-dependent signaling cascade mechanism, thus it may lead to establish a drug to treat type 2 diabetes mellitus.
Authors: Prawej Ansari; Samia Akther; J M A Hannan; Veronique Seidel; Nusrat Jahan Nujat; Yasser H A Abdel-Wahab Journal: Molecules Date: 2022-07-03 Impact factor: 4.927