Sergey Assonov1, Ales Fajgelj2, Jean-François Hélie3, Colin Allison4, Manfred Gröning2. 1. Formerly at the International Atomic Energy Agency (IAEA), Wagramerstrasse 5, Vienna, 1400, Austria. 2. International Atomic Energy Agency (IAEA), Wagramerstrasse 5, Vienna, 1400, Austria. 3. Université du Québec à Montréal, Montréal, H2X 3Y7, QC, Canada. 4. CSIRO, GPO Box 1700, Canberra, ACT, 2601, Australia.
Abstract
Rationale LSVEC, the second anchor Reference Material (RM) for the VPDB δ13 C scale realisation was introduced in 2006. In 2015, its δ13 C value was found to be drifting and, in 2017, its use as an RM for δ13 C was officially discontinued by IUPAC. New RMs of low uncertainty are needed. This paper describes the preparation and characterisation of IAEA-610, IAEA-611 and IAEA-612, (calcium carbonate, of chemical origin) which shall serve as a set of RMs aimed at anchoring the VPDB scale at negative δ13 C values. METHODS: The preparation and characterisation of IAEA-610, IAEA-611 and IAEA-612 were performed by addressing the contemporary technical requirements for RM production and characterisation (ISO Guide 35:2017). The three RMs were produced in a large quantity, and the first batch was sealed into ampoules (0.5 g) to ensure integrity of RM during storage; additional batches were sealed for long-term storage. The most accurate method of CO2 preparation and stable isotope measurements was used, namely carbonate-H3 PO4 reaction under well controlled conditions combined with well-tested isotope ratio mass spectrometry. RESULTS: The assigned values of δ13 C and associated uncertainties are based on a large number of analyses (~10 mg aliquots) performed at IAEA and address all the known uncertainty components. For aliquots down to 100 μg, the δ13 C uncertainty is increased. The uncertainty components considered are as follows: (i) material homogeneity, (ii) value assignment against IAEA-603, (iii) potential storage effects, (iv) effect of the 17 O correction and (v) mass-spectrometer linearity and cross-contamination memory in the ion source. CONCLUSIONS: The new RMs IAEA-610, IAEA-611 and IAEA-612 have been characterised on the VPDB δ13 C scale in a mutually consistent way. The use of three RMs will allow a consistent realisation of the VPDB δ13 C scale with small uncertainty to be established, and to reach metrological compatibility of measurement results over several decades. This article is protected by copyright. All rights reserved.
Rationale LSVEC, the second anchor Reference Material (RM) for the VPDB δ13 C scale realisation was introduced in 2006. In 2015, its δ13 C value was found to be drifting and, in 2017, its use as an RM for δ13 C was officially discontinued by IUPAC. New RMs of low uncertainty are needed. This paper describes the preparation and characterisation of IAEA-610, IAEA-611 and IAEA-612, (calcium carbonate, of chemical origin) which shall serve as a set of RMs aimed at anchoring the VPDB scale at negative δ13 C values. METHODS: The preparation and characterisation of IAEA-610, IAEA-611 and IAEA-612 were performed by addressing the contemporary technical requirements for RM production and characterisation (ISO Guide 35:2017). The three RMs were produced in a large quantity, and the first batch was sealed into ampoules (0.5 g) to ensure integrity of RM during storage; additional batches were sealed for long-term storage. The most accurate method of CO2 preparation and stable isotope measurements was used, namely carbonate-H3PO4 reaction under well controlled conditions combined with well-tested isotope ratio mass spectrometry. RESULTS: The assigned values of δ13 C and associated uncertainties are based on a large number of analyses (~10 mg aliquots) performed at IAEA and address all the known uncertainty components. For aliquots down to 100 μg, the δ13 C uncertainty is increased. The uncertainty components considered are as follows: (i) material homogeneity, (ii) value assignment against IAEA-603, (iii) potential storage effects, (iv) effect of the 17 O correction and (v) mass-spectrometer linearity and cross-contamination memory in the ion source. CONCLUSIONS: The new RMs IAEA-610, IAEA-611 and IAEA-612 have been characterised on the VPDB δ13 C scale in a mutually consistent way. The use of three RMs will allow a consistent realisation of the VPDB δ13 C scale with small uncertainty to be established, and to reach metrological compatibility of measurement results over several decades. This article is protected by copyright. All rights reserved.
Authors: Michelle M G Chartrand; Juris Meija; Jean-Francois Hélie; Paul Middlestead; Malarvili Ramalingam; Azharuddin Abd Aziz; Zoltan Mester Journal: Anal Bioanal Chem Date: 2022-10-06 Impact factor: 4.478