Junyoung Kwon1, Xiang Mao2, Hyun Ah Lee3, Sangjin Oh1, Lemma Teshome Tufa4, Jun Young Choi3, Ji Eun Kim3, Chang-Yeon Kim5, Jin-Gyu Kim5, Dae Youn Hwang3, Jaebeom Lee6. 1. Department of Cogno-Mechatronics Engineering, Pusan National University, Busan 46240, Republic of Korea. 2. Department of Cogno-Mechatronics Engineering, Pusan National University, Busan 46240, Republic of Korea; State Key Laboratory of Ultrasound in Medicine and Engineering & Chongqing Key Laboratory of Biomedical Engineering, College of Biomedical Engineering, Chongqing Medical University, Chongqing 400016, China. 3. Department of Biomaterial Science, Pusan National University, Miryang 50463, Republic of Korea. 4. Department of Cogno-Mechatronics Engineering, Pusan National University, Busan 46240, Republic of Korea; Department of Chemistry, Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 34134, Republic of Korea; Department of Applied Chemistry, Adama Science and Technology University, P.O. Box 1888, Adama, Ethiopia. 5. Nano-Bio Electron Microscopy Research Group, Korea Basic Science Institute, Daejeon 34133, Republic of Korea. 6. Department of Chemistry, Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 34134, Republic of Korea. Electronic address: nanoleelab@cnu.ac.kr.
Abstract
HYPOTHESIS: Here, FePd magnetic nanoparticles (MNPs) are developed as artificial enzymes with high biocompatibility and reusability. EXPERIMENT: The nanoparticles (NPs) are synthesized in an aqueous solvent by one-pot synthesis utilizing glutathione (GSH) and cysteine (Cys) as surfactants. FINDINGS: The prepared hydrophilic FePd NPs are redispersible in water. Further, they exhibit catalytic activity for the degradation of rhodamine B (RhB), as well as for the inhibition of reactive oxygen species (ROS) production induced by H2O2, which are two- and seven-fold enhancements of their catalytic performances, respectively, compared with that of horseradish peroxidase. The computational simulation and electrochemical analysis indicate that the enhancement of the catalytic effect is due to the protection of the MNP surface by GSH and Cys. In vitro experiments reveal that FePd MNPs behave like a peroxidase and decrease the ROS in mammalian cells. The cytotoxicity assessment of FePd MNPs via exposures to different cell lines for over seven days indicates that they can maintain the cell viability of >90% for up to 20 μgmL-1 concentration. FePd MNPs with high saturation magnetization and biocompatibility can be utilized as recyclable peroxidase-mimicking nanozymes and biosensors in a variety of catalytic and biological applications.
HYPOTHESIS: Here, FePd magnetic nanoparticles (MNPs) are developed as artificial enzymes with high biocompatibility and reusability. EXPERIMENT: The nanoparticles (NPs) are synthesized in an aqueous solvent by one-pot synthesis utilizing glutathione (GSH) and cysteine (Cys) as surfactants. FINDINGS: The prepared hydrophilic FePd NPs are redispersible in water. Further, they exhibit catalytic activity for the degradation of rhodamine B (RhB), as well as for the inhibition of reactive oxygen species (ROS) production induced by H2O2, which are two- and seven-fold enhancements of their catalytic performances, respectively, compared with that of horseradish peroxidase. The computational simulation and electrochemical analysis indicate that the enhancement of the catalytic effect is due to the protection of the MNP surface by GSH and Cys. In vitro experiments reveal that FePd MNPs behave like a peroxidase and decrease the ROS in mammalian cells. The cytotoxicity assessment of FePd MNPs via exposures to different cell lines for over seven days indicates that they can maintain the cell viability of >90% for up to 20 μgmL-1 concentration. FePd MNPs with high saturation magnetization and biocompatibility can be utilized as recyclable peroxidase-mimicking nanozymes and biosensors in a variety of catalytic and biological applications.