Gerard Baiges-Gaya1, Salvador Fernández-Arroyo1, Fedra Luciano-Mateo1, Noemí Cabré1, Elisabet Rodríguez-Tomàs1, Anna Hernández-Aguilera1, Helena Castañé1, Marta Romeu2, Maria-Rosa Nogués2, Jordi Camps3, Jorge Joven4. 1. Universitat Rovira i Virgili, Departament de Medicina i Cirurgia, Facultat de Medicina, Reus, Spain; Unitat de Recerca Biomèdica, Hospital Universitari de Sant Joan, Institut d'investigació Sanitària Pere Virgili, Reus, Spain. 2. Universitat Rovira i Virgili, Departament de Ciències Mèdiques Bàsiques, Facultat de Medicina, Unitat de Farmacologia, Reus, Spain. 3. Universitat Rovira i Virgili, Departament de Medicina i Cirurgia, Facultat de Medicina, Reus, Spain; Unitat de Recerca Biomèdica, Hospital Universitari de Sant Joan, Institut d'investigació Sanitària Pere Virgili, Reus, Spain. Electronic address: jcamps@grupsagessa.com. 4. Universitat Rovira i Virgili, Departament de Medicina i Cirurgia, Facultat de Medicina, Reus, Spain; Unitat de Recerca Biomèdica, Hospital Universitari de Sant Joan, Institut d'investigació Sanitària Pere Virgili, Reus, Spain; Campus of International Excellence Southern Catalonia, Tarragona, Spain. Electronic address: jjoven@grupsagessa.com.
Abstract
BACKGROUND: Obesity is a chronic progressive disease with several metabolic alterations. Nonalcoholic fatty liver disease (NAFLD) is an important comorbidity of obesity that can progress to nonalcoholic steatohepatitis (NASH), cirrhosis or hepatocarcinoma. This study aimed at clarifying the molecular mechanisms underlying the metabolic alterations in hepatic and adipose tissue during high-fat high-sucrose diet-induced NAFLD development in mice. METHODS: Twenty-four male mice (C57BL/6J) were randomly allocated into 3 groups (n = 8 mice per group) to receive a chow diet, a high-fat diet (HFD), or a high-fat high-sucrose diet (HF-HSD) for 20 weeks. At sacrifice, liver and adipose tissue were obtained for histopathological, metabolomic, and protein expression analyses. RESULTS: HF-HSD (but not HFD) was associated with NASH and increased oxidative stress. These animals presented an inhibition of hepatic autophagy and alterations in AMP-activated protein kinase/mammalian target of rapamycin activity. We also observed that the ability of metabolic adaptation was adversely affected by the increase of damaged mitochondria. NASH development was associated with changes in adipose tissue dynamics and increased amounts of saturated fatty acids, monounsaturated fatty acids and polyunsaturated fatty acids in visceral adipose tissue. CONCLUSION: HF-HSD led to a metabolic blockage and impaired hepatic mitochondria turnover. In addition, the continuous accumulation of fatty acids produced adipose tissue dysfunction and hepatic fat accumulation that favored the progression to NASH.
BACKGROUND:Obesity is a chronic progressive disease with several metabolic alterations. Nonalcoholic fatty liver disease (NAFLD) is an important comorbidity of obesity that can progress to nonalcoholic steatohepatitis (NASH), cirrhosis or hepatocarcinoma. This study aimed at clarifying the molecular mechanisms underlying the metabolic alterations in hepatic and adipose tissue during high-fat high-sucrose diet-induced NAFLD development in mice. METHODS: Twenty-four male mice (C57BL/6J) were randomly allocated into 3 groups (n = 8 mice per group) to receive a chow diet, a high-fat diet (HFD), or a high-fat high-sucrose diet (HF-HSD) for 20 weeks. At sacrifice, liver and adipose tissue were obtained for histopathological, metabolomic, and protein expression analyses. RESULTS:HF-HSD (but not HFD) was associated with NASH and increased oxidative stress. These animals presented an inhibition of hepatic autophagy and alterations in AMP-activated protein kinase/mammalian target of rapamycin activity. We also observed that the ability of metabolic adaptation was adversely affected by the increase of damaged mitochondria. NASH development was associated with changes in adipose tissue dynamics and increased amounts of saturated fatty acids, monounsaturated fatty acids and polyunsaturated fatty acids in visceral adipose tissue. CONCLUSION:HF-HSD led to a metabolic blockage and impaired hepatic mitochondria turnover. In addition, the continuous accumulation of fatty acids produced adipose tissue dysfunction and hepatic fat accumulation that favored the progression to NASH.
Authors: Helena Castañé; Simona Iftimie; Gerard Baiges-Gaya; Elisabet Rodríguez-Tomàs; Andrea Jiménez-Franco; Ana Felisa López-Azcona; Pedro Garrido; Antoni Castro; Jordi Camps; Jorge Joven Journal: Metabolism Date: 2022-04-02 Impact factor: 13.934
Authors: Aina Lluch; Sonia R Veiga; Jèssica Latorre; José M Moreno-Navarrete; Núria Bonifaci; Van Dien Nguyen; You Zhou; Marcus Höring; Gerhard Liebisch; Vesa M Olkkonen; David Llobet-Navas; George Thomas; Ruth Rodríguez-Barrueco; José M Fernández-Real; Sara C Kozma; Francisco J Ortega Journal: JCI Insight Date: 2022-07-22