Anders Öman1, Johan Dicksved1, Lars Engstrand1, Lillemor Berntson1. 1. The study was supported by grants from the Uppsala-Örebro Regional Research Council, the Gillbergska Foundation Uppsala, the Samariten Foundation for Paediatric Research, and the Swedish Rheumatism Association. 1A. Öman, MD, L. Berntson, MD, Associate Professor, Department of Women's and Children's Health, Uppsala University, Uppsala; 2J. Dicksved, PhD, Associate Professor, Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, Uppsala; 3L. Engstrand, MD, Professor, Center for Translational Microbiome Research, CTMR, Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Science for Life Laboratory, Solna, Sweden. The authors have declared no conflicts of interest. Address correspondence to Dr. A. Öman, Unit for Pediatric Rheumatology, Department of Pediatrics, Uppsala University Hospital, S-75185 Uppsala, Sweden. Email: anders.oman@kbh.uu.se. Accepted for publication November 11, 2020.
Abstract
OBJECTIVE: Changes in the composition of gut microbiota have been suggested to be associated with juvenile idiopathic arthritis ( JIA). The objective in this study was to investigate if the diversity and composition of the fecal microbiota differed between children with JIA and healthy controls (HCs), and if the microbiota differed between children with JIA and their healthy siblings. METHODS: In this multicenter, case-control study, fecal samples were collected from 75 children with JIA and 32 HCs. Eight of the HCs were siblings to 8 children with JIA, and they were compared only pairwise with their siblings. The microbiota was determined using sequencing amplicons from the V3 and V4 regions of the 16S rRNA gene. Alpha diversity, community composition of microbiota, and relative abundances of taxa were compared between children with JIA and healthy unrelated controls as well as between children with JIA and healthy siblings. RESULTS: Our data revealed no significant differences in α-diversity or community composition of microbiota between children with JIA, healthy unrelated controls, or healthy siblings. Analyses of relative abundances of phyla, families, and genera identified trends of differing abundances of some taxa in children with JIA, in comparison with both HCs and healthy siblings, but none of these findings were significant after adjustment for multiple comparisons. CONCLUSION: There were no significant differences in the composition of fecal microbiota in children with JIA compared with HCs. The composition of microbiota in children with JIA did not differ significantly from that in their healthy siblings.
OBJECTIVE: Changes in the composition of gut microbiota have been suggested to be associated with juvenile idiopathic arthritis ( JIA). The objective in this study was to investigate if the diversity and composition of the fecal microbiota differed between children with JIA and healthy controls (HCs), and if the microbiota differed between children with JIA and their healthy siblings. METHODS: In this multicenter, case-control study, fecal samples were collected from 75 children with JIA and 32 HCs. Eight of the HCs were siblings to 8 children with JIA, and they were compared only pairwise with their siblings. The microbiota was determined using sequencing amplicons from the V3 and V4 regions of the 16S rRNA gene. Alpha diversity, community composition of microbiota, and relative abundances of taxa were compared between children with JIA and healthy unrelated controls as well as between children with JIA and healthy siblings. RESULTS: Our data revealed no significant differences in α-diversity or community composition of microbiota between children with JIA, healthy unrelated controls, or healthy siblings. Analyses of relative abundances of phyla, families, and genera identified trends of differing abundances of some taxa in children with JIA, in comparison with both HCs and healthy siblings, but none of these findings were significant after adjustment for multiple comparisons. CONCLUSION: There were no significant differences in the composition of fecal microbiota in children with JIA compared with HCs. The composition of microbiota in children with JIA did not differ significantly from that in their healthy siblings.
Authors: Tejpal Gill; Patrick Stauffer; Mark Asquith; Ted Laderas; Tammy M Martin; Sean Davin; Matthew Schleisman; Claire Ramirez; Kimberly Ogle; Ingrid Lindquist; Justine Nguyen; Stephen R Planck; Carley Shaut; Sarah Diamond; James T Rosenbaum; Lisa Karstens Journal: Front Immunol Date: 2022-09-28 Impact factor: 8.786