Literature DB >> 3325479

Scanning microfluorometric measurement of immunofluorescently labelled microtubules in cultured cells. Dependence of microtubule content on cell density.

J Kajstura1, J Bereiter-Hahn.   

Abstract

A method for evaluation of microtubule content in cultured cells has been developed. The method is based on scanning microfluorometric measurement of immunofluorescently labelled microtubules. The method has been applied to the comparison of microtubule content in epithelial XTH-2 cells grown in culture at various cell densities. The results have shown that the microtubule content in the cells is not dependent on their proliferative state rather than it depends on cellular contacts.

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Year:  1987        PMID： 3325479     DOI： 10.1007/bf00490167

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  16 in total

1.  Determination of human factor VIII (AHG-associated protein)--antigen in endothelial cells from Xenopus laevis (XTH cells). Evaluation of a sensitive ELISA technique.

Authors:  W K Schlage
Journal:  Cell Tissue Res       Date:  1986       Impact factor: 5.249

2.  Competence of soluble cell extracts as microtubule assembly systems. Comparison of simian virus 40 transformed and nontransformed mouse 3T3 fibroblasts.

Authors:  G Wiche; V J Lundblad; R D Cole
Journal:  J Biol Chem       Date:  1977-01-25       Impact factor: 5.157

3.  Scanning microfluorometric measurement of TRITC-phalloidin labelled F-actin. Dependence of F-actin content on density of normal and transformed cells.

Authors:  J Bereiter-Hahn; J Kajstura
Journal:  Histochemistry       Date:  1988

4.  Modulation of the nuclear-envelope nucleoside triphosphatase by poly(A)-rich mRNA and by microtubule protein.

Authors:  A Bernd; H C Schröder; R K Zahn; W E Müller
Journal:  Eur J Biochem       Date:  1982-12

5.  Regulation of intracellular levels of calmodulin and tubulin in normal and transformed cells.

Authors:  J G Chafouleas; R L Pardue; B R Brinkley; J R Dedman; A R Means
Journal:  Proc Natl Acad Sci U S A       Date:  1981-02       Impact factor: 11.205

6.  Serum-stimulated release of cell contacts and the initiation of growth in contact-inhibited chick fibroblasts.

Authors:  J B Baker; T Humphreys
Journal:  Proc Natl Acad Sci U S A       Date:  1971-09       Impact factor: 11.205

7.  Significance of energy metabolism pathways for stimulation of DNA synthesis by cell migration and serum.

Authors:  J Kajstura; W Korohoda
Journal:  Eur J Cell Biol       Date:  1983-07       Impact factor: 4.492

8.  Amino acid sequence requirements in the epitope recognized by the alpha-tubulin-specific rat monoclonal antibody YL 1/2.

Authors:  J Wehland; H C Schröder; K Weber
Journal:  EMBO J       Date:  1984-06       Impact factor: 11.598

9.  Platelet-derived growth factor-induced alterations in vinculin and actin distribution in BALB/c-3T3 cells.

Authors:  B Herman; W J Pledger
Journal:  J Cell Biol       Date:  1985-04       Impact factor: 10.539

10.  Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line.

Authors:  J V Kilmartin; B Wright; C Milstein
Journal:  J Cell Biol       Date:  1982-06       Impact factor: 10.539
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  2 in total

1.  Scanning microfluorometric measurement of cell constituents. Principles of the method and its application to the determination of NAD content and redox state of XTH-2 cells in culture.

Authors:  J Kajstura; J Bereiter-Hahn
Journal:  Histochemistry       Date:  1988

2.  Solute-dependent activation of cell motility in strongly hypertonic solutions in Dictyostelium discoideum, human melanoma HTB-140 cells and walker 256 carcinosarcoma cells.

Authors:  Włodzimierz Korohoda; Magdalena Kucia; Ewa Wybieralska; Magdalena Wianecka-Skoczeń; Agnieszka Waligórska; Justyna Drukała; Zbigniew Madeja
Journal:  Cell Mol Biol Lett       Date:  2011-05-25       Impact factor: 5.787
  2 in total

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