Luisa Galati1, Rosario N Brancaccio1, Purnima Gupta1, Eugenie Lohmann1, Alexis Robitaille1, Racheal S Dube Mandishora2, Cyrille Cuenin1, Raffaele Filotico3, Jean-Damien Combes1, Anna R Giuliano4, Maria Gabriella Donà5, Massimo Tommasino6, Tarik Gheit7. 1. International Agency for Research on Cancer, 150 cours Albert Thomas, 69372 Lyon Cedex 08, France. 2. International Agency for Research on Cancer, 150 cours Albert Thomas, 69372 Lyon Cedex 08, France; Department of Medical Microbiology, University of Zimbabwe College of Health Sciences, Harare, Zimbabwe. 3. Dermatologia Oncologica, IRCCS G. Paolo II, Istituto Tumori, Bari, Italy. 4. Center for Immunization and Infection Research in Cancer, Moffitt Cancer Center, Tampa, FL, USA. 5. STI/HIV Unit, San Gallicano Dermatological Institute IRCCS, Rome, Italy. 6. International Agency for Research on Cancer, 150 cours Albert Thomas, 69372 Lyon Cedex 08, France. Electronic address: tommasinom@iarc.fr. 7. International Agency for Research on Cancer, 150 cours Albert Thomas, 69372 Lyon Cedex 08, France. Electronic address: gheitt@iarc.fr.
Abstract
BACKGROUND: To characterize the HPV diversity in the anal mucosa of men with different sexual behavior and HIV status by next-generation sequencing (NGS). METHODS: Anal swabs from HIV-positive (n = 94; mean age, 38 years) and HIV-negative (n = 100; mean age, 37.5 years) men who have sex with men (MSM) and HIV-negative men (predominantly men who have sex with women, MSW) (n = 99; mean age, 38.2 years) were analyzed by broad-spectrum PCR protocols combined with NGS. FINDINGS: Alpha HPV types (n = 74) were detected mainly in the MSM groups (HPV6, 11, and 43 were the most abundant types) compared with MSW (n = 16) (HPV11, 32, and 87 were among the most abundant). In contrast, beta HPVs were more abundantly detected among MSW (n = 45) than in the HIV-positive (n = 16) and HIV-negative (n = 26) MSM groups. Gamma HPVs were detected almost equally in HIV-positive MSM (n = 62), HIV-negative MSM (n = 58), and MSW (n = 57). In addition, 31 putative novel PV types were identified. CONCLUSIONS: Our data show that beta and gamma HPV types are present in the anal mucosa, thus reinforcing the existing evidence that they can be detected at anatomical sites other than skin. Alpha and beta HPV distribution among these three groups appears to vary according to sexual behavior.
BACKGROUND: To characterize the HPV diversity in the anal mucosa of men with different sexual behavior and HIV status by next-generation sequencing (NGS). METHODS: Anal swabs from HIV-positive (n = 94; mean age, 38 years) and HIV-negative (n = 100; mean age, 37.5 years) men who have sex with men (MSM) and HIV-negative men (predominantly men who have sex with women, MSW) (n = 99; mean age, 38.2 years) were analyzed by broad-spectrum PCR protocols combined with NGS. FINDINGS: Alpha HPV types (n = 74) were detected mainly in the MSM groups (HPV6, 11, and 43 were the most abundant types) compared with MSW (n = 16) (HPV11, 32, and 87 were among the most abundant). In contrast, beta HPVs were more abundantly detected among MSW (n = 45) than in the HIV-positive (n = 16) and HIV-negative (n = 26) MSM groups. Gamma HPVs were detected almost equally in HIV-positive MSM (n = 62), HIV-negative MSM (n = 58), and MSW (n = 57). In addition, 31 putative novel PV types were identified. CONCLUSIONS: Our data show that beta and gamma HPV types are present in the anal mucosa, thus reinforcing the existing evidence that they can be detected at anatomical sites other than skin. Alpha and beta HPV distribution among these three groups appears to vary according to sexual behavior.