Nanodiamonds and hydrogen-substituted graphdiyne heteronanostructure for the sensitive impedimetric aptasensing of myocardial infarction and cardiac troponin I.

A novel heteronanostructure of nanodiamonds (NDs) and hydrogen-substituted graphdiyne (HsGDY) (denoted as HsGDY@NDs) was prepared for the impedimetric aptasensing of biomarkers such as myoglobin (Myo) and cardiac troponin I (cTnI). Basic characterizations revealed that the HsGDY@NDs were composed of nanospheres with sizes of 200-500 nm. In these nanospheres, NDs were embedded within the HsGDY network. The HsGDY@NDs nanostructure, which integrated the good chemical stability and three-dimensional porous networks of HsGDY, and the good biocompatibility and electrochemical activity of NDs, could immobilize diverse aptamer strands and recognize target biomarkers. Compared with HsGDY- and NDs-based aptasensors, the HsGDY@NDs-based aptasensors exhibited superior sensing performances for Myo and cTnI, giving low detection limits of 6.29 and 9.04 fg mL-1 for cTnI and Myo, respectively. In addition, the HsGDY@NDs-based aptasensors exhibited high selectivity, good stability, reproducibility, and acceptable applicability in real human serum. Thus, the construction of HsGDY@NDs-based aptasensor is expected to broaden the application of porous organic frameworks in the sensing field and provide a prospective approach for the early detection of disease biomarkers.