Major challenges in cryopreservation of sea urchin eggs.

Cryopreservation of gametes, embryos and larvae of marine invertebrates has been investigated in many studies throughout the years. There are many favorable studies on sperm cryopreservation but oocytes are still under research as no successful results have been sustainably obtained for this type of cells. The preservation of both maternal and paternal gametes separately would provide a reliable source of genetic material for their application to conservation, aquaculture and fundamental research. Unfortunately to date, it has not been possible to cryopreserve eggs from marine organisms. The aim of this review is to go over the factors that have been historically considered as obstacles for oocyte cryopreservation in aquatic organisms and discern those that may specifically apply to eggs of the sea urchin Paracentrotus lividus.