Yingchun Li1, Zheng Zhao2, Dan Sun1, Yanfei Li1. 1. Department of Gynaecology, CangZhou Central Hospital, Cangzhou City, China. 2. Department of Cardiology, CangZhou Central Hospital, Cangzhou City, China.
Abstract
BACKGROUND: This study was aimed at investigating the effects of long noncoding RNA (lncRNA) LINC02323 in ovarian cancer and its possible mechanism. METHODS: Microarray analysis and QPCR were utilized to identify lncRNA LINC02323 expression in patients with ovarian cancer. MTT assay was used for analysis of ovarian cancer cell proliferation. Western blot was utilized to investigate its possible mechanism. RESULTS: In patients with ovarian cancer, lncRNA LINC02323 expression was up-regulated and miR-1343-3p expression was down-regulated. Over-expression of lncRNA LINC02323 promoted cell growth and reduced LDH activity levels in vitro model by suppression of miR-1343-3p expression. Down-regulation of lncRNA LINC02323 reduced cell growth and increased LDH activity levels in vitro model by induction of miR-1343-3p expression. Over-expression of miR-1343-3p reduced cell growth and reduced LDH activity levels in vitro model by suppression of TGF-β receptor. Down-regulation of miR-1343-3p promoted cell growth and reduced LDH activity levels in vitro model by induced of TGF-β receptor. CONCLUSION: Our findings show that Novel long noncoding RNA LINC02323 promotes cell growth of ovarian cancer via TGF-β receptor 1 by miR-1343-3p.
BACKGROUND: This study was aimed at investigating the effects of long noncoding RNA (lncRNA) LINC02323 in ovarian cancer and its possible mechanism. METHODS: Microarray analysis and QPCR were utilized to identify lncRNA LINC02323 expression in patients with ovarian cancer. MTT assay was used for analysis of ovarian cancer cell proliferation. Western blot was utilized to investigate its possible mechanism. RESULTS: In patients with ovarian cancer, lncRNA LINC02323 expression was up-regulated and miR-1343-3p expression was down-regulated. Over-expression of lncRNA LINC02323 promoted cell growth and reduced LDH activity levels in vitro model by suppression of miR-1343-3p expression. Down-regulation of lncRNA LINC02323 reduced cell growth and increased LDH activity levels in vitro model by induction of miR-1343-3p expression. Over-expression of miR-1343-3p reduced cell growth and reduced LDH activity levels in vitro model by suppression of TGF-β receptor. Down-regulation of miR-1343-3p promoted cell growth and reduced LDH activity levels in vitro model by induced of TGF-β receptor. CONCLUSION: Our findings show that Novel long noncoding RNA LINC02323 promotes cell growth of ovarian cancer via TGF-β receptor 1 by miR-1343-3p.