Laura E Levin1, Leila H Shayegan2, Anne W Lucky3, Kristen P Hook4, Anna L Bruckner5, James A Feinstein6, Susan Whittier7, Christine T Lauren8, Elena Pope9, Irene Lara-Corrales9, Karen Wiss10, Catherine C McCuaig11, Julie Powell12, Lawrence F Eichenfield13, Moise L Levy14, Lucia Diaz15, Sharon A Glick16, Amy S Paller17, Harper N Price18, John C Browning19, Kimberly D Morel8. 1. Department of Dermatology, Columbia University Irving Medical Center, New York, NY, USA. 2. Columbia University Vagelos College of Physicians and Surgeons, New York, NY, USA. 3. Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA. 4. Department of Dermatology, University of Minnesota Medical School, Minneapolis, MN, USA. 5. Departments of Dermatology and Pediatrics, University of Colorado School of Medicine, Aurora, CO, USA. 6. Departments of Pediatrics, University of Colorado School of Medicine, Aurora, CO, USA. 7. Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York, NY, USA. 8. Departments of Pediatrics and Dermatology, Columbia University Irving Medical Center, New York, NY, USA. 9. Section of Dermatology, Division of Paediatric Medicine, Hospital for Sick Children, Toronto, ON, Canada. 10. Division of Dermatology, Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA. 11. Departments of Pediatrics and Dermatology, CHU Sainte-Justine, University of Montreal, Montreal, QC, Canada. 12. Department of Dermatology, CHU Sainte-Justine, University of Montreal, Montreal, QC, Canada. 13. Departments of Pediatrics and Dermatology, University of California San Diego, San Diego, CA, USA. 14. Departments of Pediatrics and Dermatology, Dell Children's Medical Center, Austin, TX, USA. 15. Department of Dermatology, Dell Children's Medical Center, Austin, TX, USA. 16. Department of Dermatology, State University of New York Downstate Medical Center, Brooklyn, NY, USA. 17. Departments of Pediatrics and Dermatology, Northwestern University, Chicago, IL, USA. 18. Department of Dermatology, Phoenix Children's Hospital, Phoenix, AZ, USA. 19. Department of Dermatology, The Children's Hospital of San Antonio, San Antonio, TX, USA.
Abstract
BACKGROUND/ OBJECTIVES: Patients with epidermolysis bullosa (EB) require care of wounds that are colonized or infected with bacteria. A subset of EB patients are at risk for squamous cell carcinoma, and bacterial-host interactions have been considered in this risk. The EB Clinical Characterization and Outcomes Database serves as a repository of information from EB patients at multiple centers in the United States and Canada. Access to this resource enabled broad-scale analysis of wound cultures. METHODS: A retrospective analysis of 739 wound cultures from 158 patients from 13 centers between 2001 and 2018. RESULTS: Of 152 patients with a positive culture, Staphylococcus aureus (SA) was recovered from 131 patients (86%), Pseudomonas aeruginosa (PA) from 56 (37%), and Streptococcus pyogenes (GAS) from 34 (22%). Sixty-eight percent of patients had cultures positive for methicillin-sensitive SA, and 47%, methicillin-resistant SA (18 patients had cultures that grew both methicillin-susceptible and methicillin-resistant SA at different points in time). Of 15 patients with SA-positive cultures with recorded mupirocin susceptibility testing, 11 had mupirocin-susceptible SA and 6 patients mupirocin-resistant SA (2 patients grew both mupirocin-susceptible and mupirocin-resistant SA). SCC was reported in 23 patients in the entire database, of whom 10 had documented wound cultures positive for SA, PA, and Proteus species in 90%, 50%, and 20% of cases, respectively. CONCLUSIONS: SA and PA were the most commonly isolated bacteria from wounds. Methicillin resistance and mupirocin resistance were reported in 47% and 40% of patients tested, respectively, highlighting the importance of ongoing antimicrobial strategies to limit antibiotic resistance.
BACKGROUND/ OBJECTIVES: Patients with epidermolysis bullosa (EB) require care of wounds that are colonized or infected with bacteria. A subset of EB patients are at risk for squamous cell carcinoma, and bacterial-host interactions have been considered in this risk. The EB Clinical Characterization and Outcomes Database serves as a repository of information from EB patients at multiple centers in the United States and Canada. Access to this resource enabled broad-scale analysis of wound cultures. METHODS: A retrospective analysis of 739 wound cultures from 158 patients from 13 centers between 2001 and 2018. RESULTS: Of 152 patients with a positive culture, Staphylococcus aureus (SA) was recovered from 131 patients (86%), Pseudomonas aeruginosa (PA) from 56 (37%), and Streptococcus pyogenes (GAS) from 34 (22%). Sixty-eight percent of patients had cultures positive for methicillin-sensitive SA, and 47%, methicillin-resistant SA (18 patients had cultures that grew both methicillin-susceptible and methicillin-resistant SA at different points in time). Of 15 patients with SA-positive cultures with recorded mupirocin susceptibility testing, 11 had mupirocin-susceptible SA and 6 patients mupirocin-resistant SA (2 patients grew both mupirocin-susceptible and mupirocin-resistant SA). SCC was reported in 23 patients in the entire database, of whom 10 had documented wound cultures positive for SA, PA, and Proteus species in 90%, 50%, and 20% of cases, respectively. CONCLUSIONS: SA and PA were the most commonly isolated bacteria from wounds. Methicillin resistance and mupirocin resistance were reported in 47% and 40% of patients tested, respectively, highlighting the importance of ongoing antimicrobial strategies to limit antibiotic resistance.
Authors: Nina K Antonov; Maria C Garzon; Kimberly D Morel; Susan Whittier; Paul J Planet; Christine T Lauren Journal: Antimicrob Agents Chemother Date: 2015-03-30 Impact factor: 5.191
Authors: Magdalena M van der Kooi-Pol; José C Duipmans; Marcel F Jonkman; Jan Maarten van Dijl Journal: Int J Med Microbiol Date: 2013-12-01 Impact factor: 3.473
Authors: James A Feinstein; Purevsuren Jambal; Kathleen Peoples; Anne W Lucky; Phuong Khuu; Jean Y Tang; Irene Lara-Corrales; Elena Pope; Karen Wiss; Kristen P Hook; Laura E Levin; Kimberly D Morel; Amy S Paller; Catherine C McCuaig; Julie Powell; Lawrence F Eichenfield; Harper Price; Moise L Levy; Lawrence A Schachner; John C Browning; Susan Bayliss; Marla Jahnke; Tor Shwayder; Sharon A Glick; Anna L Bruckner Journal: JAMA Dermatol Date: 2019-02-01 Impact factor: 10.282
Authors: Hannah M Singer; Laura E Levin; Maria C Garzon; Christine T Lauren; Paul J Planet; Nicole W Kittler; Susan Whittier; Kimberly D Morel Journal: Pediatr Dermatol Date: 2017-11-06 Impact factor: 1.588
Authors: Leila H Shayegan; Laura E Levin; Eloise R Galligan; Anne W Lucky; Anna L Bruckner; Elena Pope; Irene Lara-Corrales; Karen Wiss; Catherine C McCuaig; Maria C Garzon; Lawrence F Eichenfield; Kristen P Hook; John C Browning; Lawrence A Schachner; Marissa J Perman; Leslie Castelo-Soccio; Moise L Levy; Sharon A Glick; Kimberly D Morel Journal: Pediatr Dermatol Date: 2020-01-15 Impact factor: 1.588
Authors: Esther Hoste; Esther N Arwert; Rohit Lal; Andrew P South; Julio C Salas-Alanis; Dedee F Murrell; Giacomo Donati; Fiona M Watt Journal: Nat Commun Date: 2015-01-09 Impact factor: 14.919
Authors: Nadezhda A Evtushenko; Arkadii K Beilin; Anastasiya V Kosykh; Ekaterina A Vorotelyak; Nadya G Gurskaya Journal: Int J Mol Sci Date: 2021-11-18 Impact factor: 5.923