INTRODUCTION: Freshwater ecosystems provide propitious conditions for the acquisition and spread of antibiotic resistance genes (ARGs), and integrons play an important role in this process. MATERIAL AND METHODS: In the present study, the diversity of putative environmental integron-cassettes, as well as their potential bacterial hosts in the Velhas River (Brazil), was explored through intI-attC and 16S rRNA amplicons deep sequencing. RESULTS AND DISCUSSION: ORFs related to different biological processes were observed, from DNA integration to oxidation-reduction. ARGs-cassettes were mainly associated with class 1 mobile integrons carried by pathogenic Gammaproteobacteria, and possibly sedentary chromosomal integrons hosted by Proteobacteria and Actinobacteria. Two putative novel ARG-cassettes homologs to fosB3 and novA were detected. Regarding 16SrRNA gene analysis, taxonomic and functional profiles unveiled Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria as dominant phyla. Betaproteobacteria, Alphaproteobacteria, and Actinobacteria classes were the main contributors for KEGG orthologs associated with resistance. CONCLUSIONS: Overall, these results provide new information about environmental integrons as a source of resistance determinants outside clinical settings and the bacterial community in the Velhas River.
INTRODUCTION: Freshwater ecosystems provide propitious conditions for the acquisition and spread of antibiotic resistance genes (ARGs), and integrons play an important role in this process. MATERIAL AND METHODS: In the present study, the diversity of putative environmental integron-cassettes, as well as their potential bacterial hosts in the Velhas River (Brazil), was explored through intI-attC and 16S rRNA amplicons deep sequencing. RESULTS AND DISCUSSION: ORFs related to different biological processes were observed, from DNA integration to oxidation-reduction. ARGs-cassettes were mainly associated with class 1 mobile integrons carried by pathogenic Gammaproteobacteria, and possibly sedentary chromosomal integrons hosted by Proteobacteria and Actinobacteria. Two putative novel ARG-cassettes homologs to fosB3 and novA were detected. Regarding 16SrRNA gene analysis, taxonomic and functional profiles unveiled Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria as dominant phyla. Betaproteobacteria, Alphaproteobacteria, and Actinobacteria classes were the main contributors for KEGG orthologs associated with resistance. CONCLUSIONS: Overall, these results provide new information about environmental integrons as a source of resistance determinants outside clinical settings and the bacterial community in the Velhas River.
Authors: Yongfei Hu; Xi Yang; Jing Li; Na Lv; Fei Liu; Jun Wu; Ivan Y C Lin; Na Wu; Bart C Weimer; George F Gao; Yulan Liu; Baoli Zhu Journal: Appl Environ Microbiol Date: 2016-10-27 Impact factor: 4.792
Authors: Peter J Collignon; John M Conly; Antoine Andremont; Scott A McEwen; Awa Aidara-Kane; Yvonne Agerso; Antoine Andremont; Peter Collignon; John Conly; Tran Dang Ninh; Pilar Donado-Godoy; Paula Fedorka-Cray; Heriberto Fernandez; Marcelo Galas; Rebecca Irwin; Beth Karp; Gassan Matar; Patrick McDermott; Scott McEwen; Eric Mitema; Richard Reid-Smith; H Morgan Scott; Ruby Singh; Caroline Smith DeWaal; John Stelling; Mark Toleman; Haruo Watanabe; Gun-Jo Woo Journal: Clin Infect Dis Date: 2016-07-20 Impact factor: 9.079
Authors: Timothy M Ghaly; Anahit Penesyan; Alexander Pritchard; Qin Qi; Vaheesan Rajabal; Sasha G Tetu; Michael R Gillings Journal: Microb Genom Date: 2022-03