Literature DB >> 3324464

Bovine interleukin 2: cloning, high level expression, and purification.

P E Baker1.   

Abstract

We utilized a human IL2 probe to isolate bovine IL2 sequences from a lymph node cDNA library. Bovine IL2 was subsequently expressed in both bacteria and yeast. Using a rapid, two-step purification scheme, we have been able to isolate over 20 mg/l of homogenous bovine rIL2 secreted from the yeast. The availability of sizable quantities of bovine rIL2 should make it possible to ascertain potential therapeutic or prophylactic utility of this lymphokine in cattle.

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Year:  1987        PMID： 3324464     DOI： 10.1016/0165-2427(87)90140-1

Source DB:  PubMed          Journal:  Vet Immunol Immunopathol        ISSN： 0165-2427            Impact factor:   2.046


  4 in total

1.  An analysis of the growth and differentiation of B cells isolated from follicles of the ileal Peyer's patch of sheep.

Authors:  P J Griebel; W C Davis; J D Reynolds
Journal:  Immunology       Date:  1992-04       Impact factor: 7.397

2.  Molecular chimerization of Pasteurella haemolytica leukotoxin to interleukin-2: effects on cytokine and antigen function.

Authors:  H P Hughes; M Campos; A A Potter; L A Babiuk
Journal:  Infect Immun       Date:  1992-02       Impact factor: 3.441

Review 3.  Bovine adenovirus-3 as a vaccine delivery vehicle.

Authors:  Lisanework E Ayalew; Pankaj Kumar; Amit Gaba; Niraj Makadiya; Suresh K Tikoo
Journal:  Vaccine       Date:  2014-12-09       Impact factor: 3.641

4.  Characterization of bovine interleukin-2 stably expressed in HEK-293 cells.

Authors:  Shuya Mitoma; Heba M El-Khaiat; Tomofumi Uto; Katsuaki Sato; Satoshi Sekiguchi; Junzo Norimine
Journal:  J Vet Med Sci       Date:  2020-11-11       Impact factor: 1.267
  4 in total

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