Rong Deng1, Fuyun Zhang2, Fuzhen Lei1, Wenjun Liu1, Sisun Liu3, Wenhua Wang2. 1. Department of Gynecology, General Hospital of Pingxiang Mining Group CO., Ltd., Pingxiang 337003, China, China. 2. Department of Gynecology, Pingxiang People's Hospital, Pingxiang 337000, China. 3. Department of Obstetrics and Gynecology, First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
Abstract
OBJECTIVE: To explore the inhibitory effects of silencing long non-coding RNA (LncRNA) HIF1A-AS2 on epithelialmesenchymal transition (EMT) and tumor stem cell-like phenotype in cervical cancer cells. METHODS: We designed 3 shRNA constructs for silencing HIF1A-AS2 in CaSki cells, and the shRNA with the strongest interference effect was selected for subsequent experiment. CaSki cells were transfected with shRNA-NC or Sh-HIF1A-AS2, and the changes in cell viability, invasion ability, EMT, expressions of EMT-related proteins, formation of cell spheres and expressions of stem cell markers were detected. RESULTS: Transfection with shRNA-NC and Sh-HIF1A-AS2 did not significantly affected the viability of CaSki cells (P > 0.05). Compared with the cells transfected with shRNA-NC, the cells transfected with Sh- HIF1A-AS2 showed significantly reduced invasion ability, expressions of vimentin N-cadherin, and cell sphere formation ability. HIF1A-AS2 silencing obviously lowered the rate of ABCG2-positive cells, significantly reduced the mRNA and protein expressions of Nanog, OCT4, and SOX2, and strongly enhanced the expression of E-cadherin in CaSki cells (P < 0.05). CONCLUSIONS: Silencing HIF1A-AS2 can inhibit proliferation, invasion and migration of cervical cancer cells in vitro.
OBJECTIVE: To explore the inhibitory effects of silencing long non-coding RNA (LncRNA) HIF1A-AS2 on epithelialmesenchymal transition (EMT) and tumor stem cell-like phenotype in cervical cancer cells. METHODS: We designed 3 shRNA constructs for silencing HIF1A-AS2 in CaSki cells, and the shRNA with the strongest interference effect was selected for subsequent experiment. CaSki cells were transfected with shRNA-NC or Sh-HIF1A-AS2, and the changes in cell viability, invasion ability, EMT, expressions of EMT-related proteins, formation of cell spheres and expressions of stem cell markers were detected. RESULTS: Transfection with shRNA-NC and Sh-HIF1A-AS2 did not significantly affected the viability of CaSki cells (P > 0.05). Compared with the cells transfected with shRNA-NC, the cells transfected with Sh- HIF1A-AS2 showed significantly reduced invasion ability, expressions of vimentin N-cadherin, and cell sphere formation ability. HIF1A-AS2 silencing obviously lowered the rate of ABCG2-positive cells, significantly reduced the mRNA and protein expressions of Nanog, OCT4, and SOX2, and strongly enhanced the expression of E-cadherin in CaSki cells (P < 0.05). CONCLUSIONS: Silencing HIF1A-AS2 can inhibit proliferation, invasion and migration of cervical cancer cells in vitro.
Authors: P Bronsert; K Enderle-Ammour; M Bader; S Timme; M Kuehs; A Csanadi; G Kayser; I Kohler; D Bausch; J Hoeppner; U T Hopt; T Keck; E Stickeler; B Passlick; O Schilling; C P Reiss; Y Vashist; T Brabletz; J Berger; J Lotz; J Olesch; M Werner; U F Wellner Journal: J Pathol Date: 2014-11 Impact factor: 7.996
Authors: Marco Mineo; Franz Ricklefs; Arun K Rooj; Shawn M Lyons; Pavel Ivanov; Khairul I Ansari; Ichiro Nakano; E Antonio Chiocca; Jakub Godlewski; Agnieszka Bronisz Journal: Cell Rep Date: 2016-06-02 Impact factor: 9.423