Weiwei Wang1,2, Yuanhong Xu1. 1. Department of Clinical Laboratory, First Affiliated Hospital of Anhui Medical University, Hefei 230022, China. 2. Department of Clinical Laboratory, Fuyang People's Hospital, Fuyang Clinical College, Anhui Medical University, Fuyang 236000, China.
Abstract
OBJECTIVE: To investigate the characteristics of immunophenotypes and expressions of non-myeloid differentiation antigens in acute myeloid leukemia (AML) and their value in diagnosis and prognostic evaluation of AML. METHODS: We examined the immunophenotypes of 109 patients with AML using BD FACSCalibur flow cytometry and analyzed the association of the immunophenotypes and expressions of non-myeloid differentiation antigens with the prognosis and complete remission (CR) rate of the patients. RESULTS: Immunophenotype analysis showed that the positivity rates of the myeloid differentiation antigens of AML cells decreased in the order of CD13, CD117, CD33, MPO and CD15; the positivity rates of CD117, CD13, CD33 and MPO did not differ significantly (P > 0.05) and were all significantly higher than that of CD15 (P < 0.05). The positivity rates of AML cell non-lineage antigens CD34, CD38, HLA-DR, and CD123 did not differ significantly (P > 0.05). The positivity rates of non-myeloid differentiation antigens decreased in the order of CD9, CD200, CD56 and CD7 in AML cells and were all significantly higher than those of CD25, CD19, CD2, CD10, CD4, CyCD79a and CyCD3 (P < 0.05). Among the 109 AML patients, the CR rates of patients positive for CD7, CD34, CD56 and CD25 were significantly lower than those negative for these antigens (P < 0.05); the CR rates were significantly higher in patients positive for MPO and CD19 than in the negative patients (P < 0.05). Among the 15 AML-M2b patients with AML1-ETO positivity, the CR rate following a single treatment course was significantly lower in patients positive for CD56 than in CD56-negative patients, and CD56-positive patients also had a significantly higher relapse rate within 1 year (P < 0.05). CONCLUSIONS: Immunophenotyping and analysis of non-myeloid differentiation antigens can be of great clinical significance for the diagnosis and prognostic evaluation of AML, and serve also as one of the important bases for the diagnosis and treatment of AML.
OBJECTIVE: To investigate the characteristics of immunophenotypes and expressions of non-myeloid differentiation antigens in acute myeloid leukemia (AML) and their value in diagnosis and prognostic evaluation of AML. METHODS: We examined the immunophenotypes of 109 patients with AML using BD FACSCalibur flow cytometry and analyzed the association of the immunophenotypes and expressions of non-myeloid differentiation antigens with the prognosis and complete remission (CR) rate of the patients. RESULTS: Immunophenotype analysis showed that the positivity rates of the myeloid differentiation antigens of AML cells decreased in the order of CD13, CD117, CD33, MPO and CD15; the positivity rates of CD117, CD13, CD33 and MPO did not differ significantly (P > 0.05) and were all significantly higher than that of CD15 (P < 0.05). The positivity rates of AML cell non-lineage antigens CD34, CD38, HLA-DR, and CD123 did not differ significantly (P > 0.05). The positivity rates of non-myeloid differentiation antigens decreased in the order of CD9, CD200, CD56 and CD7 in AML cells and were all significantly higher than those of CD25, CD19, CD2, CD10, CD4, CyCD79a and CyCD3 (P < 0.05). Among the 109 AMLpatients, the CR rates of patients positive for CD7, CD34, CD56 and CD25 were significantly lower than those negative for these antigens (P < 0.05); the CR rates were significantly higher in patients positive for MPO and CD19 than in the negative patients (P < 0.05). Among the 15 AML-M2b patients with AML1-ETO positivity, the CR rate following a single treatment course was significantly lower in patients positive for CD56 than in CD56-negative patients, and CD56-positive patients also had a significantly higher relapse rate within 1 year (P < 0.05). CONCLUSIONS: Immunophenotyping and analysis of non-myeloid differentiation antigens can be of great clinical significance for the diagnosis and prognostic evaluation of AML, and serve also as one of the important bases for the diagnosis and treatment of AML.
Authors: Jun-Ting Lv; Zhi-Gang Yang; You-Hong Guang; Zhong-Shun Lin; Xing-Xian Xiao; Man Shi; Wen-Shan Wang Journal: Zhongguo Shi Yan Xue Ye Xue Za Zhi Date: 2018-06
Authors: Thomas M Maenhout; Elisabeth Moreau; Inge Van Haute; Stefanie Desmet; Dries Deeren Journal: Am J Clin Pathol Date: 2015-08 Impact factor: 2.493