| Literature DB >> 33242410 |
Sherina Malkani1, Christopher R Chin2, Egle Cekanaviciute3, Marie Mortreux4, Hazeem Okinula5, Marcel Tarbier6, Ann-Sofie Schreurs7, Yasaman Shirazi-Fard3, Candice G T Tahimic7, Deyra N Rodriguez8, Brittany S Sexton8, Daniel Butler2, Akanksha Verma2, Daniela Bezdan9, Ceyda Durmaz2, Matthew MacKay2, Ari Melnick10, Cem Meydan2, Sheng Li11, Francine Garrett-Bakelman12, Bastian Fromm6, Ebrahim Afshinnekoo13, Brad W Langhorst8, Eileen T Dimalanta8, Margareth Cheng-Campbell14, Elizabeth Blaber15, Jonathan C Schisler16, Charles Vanderburg17, Marc R Friedländer6, J Tyson McDonald18, Sylvain V Costes3, Seward Rutkove4, Peter Grabham5, Christopher E Mason19, Afshin Beheshti20.
Abstract
We have identified and validated a spaceflight-associated microRNA (miRNA) signature that is shared by rodents and humans in response to simulated, short-duration and long-duration spaceflight. Previous studies have identified miRNAs that regulate rodent responses to spaceflight in low-Earth orbit, and we have confirmed the expression of these proposed spaceflight-associated miRNAs in rodents reacting to simulated spaceflight conditions. Moreover, astronaut samples from the NASA Twins Study confirmed these expression signatures in miRNA sequencing, single-cell RNA sequencing (scRNA-seq), and single-cell assay for transposase accessible chromatin (scATAC-seq) data. Additionally, a subset of these miRNAs (miR-125, miR-16, and let-7a) was found to regulate vascular damage caused by simulated deep space radiation. To demonstrate the physiological relevance of key spaceflight-associated miRNAs, we utilized antagomirs to inhibit their expression and successfully rescue simulated deep-space-radiation-mediated damage in human 3D vascular constructs.Entities:
Keywords: NASA; Twins Study; antagomirs; miRNA-seq; microRNA; microgravity; scATAC-seq; scRNA-seq; space radiation; spaceflight
Year: 2020 PMID： 33242410 DOI： 10.1016/j.celrep.2020.108448
Source DB: PubMed Journal: Cell Rep Impact factor: 9.423