Yanmei Feng1, Qian Li1, Lan Yang1, Yu Zhang1, Daiyu Qiu1.
Abstract
OBJECTIVE: The goal of this work was to establish a method to identify and quantify the main active components in Angelicae Pubescentis Radix (APR) quickly, simply, and accurately. This paper reports a novel method which can determine osthol, isoimperatorin, and columbianadin using 1H-qNMR simultaneously and quantitatively.
METHODS: In comprehensive consideration of resolution of target signals and the solubility of materials, dimethyl sulfoxide-d6 (DMSO-d6) was selected as an optimal 1H-qNMR solvent and pyrazine was used as internal standard substance (δ8.66 ppm). The quantitative peaks of three active components were determined using specific 1H resonances at δ7.54-7.56 ppm for osthol, δ6.83-6.85 ppm for columbianadin, and δ6.31-6.32 ppm for isoimperatorin.
RESULTS: The results show that the method has good precision, stability, and repeatability. The content of APR plant material from Huating is 9.8 mg/g, 5.6 mg/g, and 15.6 mg/g for osthol, columbianadin, and isoimperatorin, respectively. Furthermore, the experimental process is simple and the test time is short (1 min).
CONCLUSIONS: The proposed quantitative 1H-qNMR methodology can be used for the quality control of APR. © AOAC INTERNATIONAL 2020. All rights reserved. For permissions, please email: journals.permissions@oup.com.
OBJECTIVE: The goal of this work was to establish a method to identify and quantify the main active components in Angelicae Pubescentis Radix (APR) quickly, simply, and accurately. This paper reports a novel method which can determine osthol, isoimperatorin, and columbianadin using 1H-qNMR simultaneously and quantitatively.
METHODS: In comprehensive consideration of resolution of target signals and the solubility of materials, dimethyl sulfoxide-d6 (DMSO-d6) was selected as an optimal 1H-qNMR solvent and pyrazine was used as internal standard substance (δ8.66 ppm). The quantitative peaks of three active components were determined using specific 1H resonances at δ7.54-7.56 ppm for osthol, δ6.83-6.85 ppm for columbianadin, and δ6.31-6.32 ppm for isoimperatorin.
RESULTS: The results show that the method has good precision, stability, and repeatability. The content of APR plant material from Huating is 9.8 mg/g, 5.6 mg/g, and 15.6 mg/g for osthol, columbianadin, and isoimperatorin, respectively. Furthermore, the experimental process is simple and the test time is short (1 min).
CONCLUSIONS: The proposed quantitative 1H-qNMR methodology can be used for the quality control of APR. © AOAC INTERNATIONAL 2020. All rights reserved. For permissions, please email: journals.permissions@oup.com.
Entities:
Mesh：
Substances：
Year: 2020
PMID： 33241377 DOI： 10.1093/jaoacint/qsz031
Source DB: PubMed Journal: J AOAC Int ISSN： 1060-3271 Impact factor: 1.913