Literature DB >> 3323810

5-Fluoroorotic acid as a selective agent in yeast molecular genetics.

J D Boeke, J Trueheart, G Natsoulis, G R Fink.   

Abstract

5-FOA is an extremely useful reagent for the selection of Ura- cells amid a population of Ura+ cells. The selection is effective in transformation and recombination studies where loss of URA3+ is desired. A new plasmid shuffling procedure based on the 5-FOAR selection permits the recovery of conditional lethal mutations in cloned genes that encode vital functions.

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Year:  1987        PMID： 3323810     DOI： 10.1016/0076-6879(87)54076-9

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  740 in total

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4.  The function of DNA polymerase alpha at telomeric G tails is important for telomere homeostasis.

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5.  Conserved bipartite motifs in yeast eIF5 and eIF2Bepsilon, GTPase-activating and GDP-GTP exchange factors in translation initiation, mediate binding to their common substrate eIF2.

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Journal:  EMBO J       Date:  1999-03-15       Impact factor: 11.598

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7.  Identification of a U2/U6 helix la mutant that influences 3' splice site selection during nuclear pre-mRNA splicing.

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Journal:  RNA       Date:  2000-08       Impact factor: 4.942

8.  A genetic system for direct selection of gene-positive clones during recombinational cloning in yeast.

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Journal:  Nucleic Acids Res       Date:  2002-01-15       Impact factor: 16.971

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Authors:  P C Liu; D J Thiele
Journal:  Mol Biol Cell       Date:  2001-11       Impact factor: 4.138

10.  Minimum requirements for the function of eukaryotic translation initiation factor 2.

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Journal:  Genetics       Date:  2001-05       Impact factor: 4.562
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