Wendong Hao1,2, Manxiang Li1, Yamei Pang1, Weiping Du3, Xiaoqi Huang4. 1. Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Xi'anJiaotong University, Xi'an, China. 2. Department of Respiratory Medicine, The Affiliated Hospital of Yan'an University, Yan'an, China. 3. Clinical Laboratory Diagnosis Department, The Affiliated Hospital of Yan'an University, Yan'an, China. 4. Department of Radiology, The Affiliated Hospital of Yan'an University, Yan'an, China.
Abstract
OBJECTIVE: We sought to explore the relationships between multiple chemokines with spirometry, inflammatory mediators and CT findings of emphysema, small airways disease and bronchial wall thickness. METHODS: All patients with COPD (n = 65) and healthy control subjects (n = 23) underwent high-resolution CT, with image analysis determining the low attenuation area (LAA), ratio of mean lung attenuation on expiratory and inspiratory scans (E/I MLD) and bronchial wall thickness of inner perimeter of a 10-mm diameter airway (Pi10). At enrollment, subjects underwent pulmonary function studies, chemokines and inflammatory mediators measurements. RESULTS: Multiple chemokines (CCL2, CCL3, CCL5, CX3CL1, CXCL8, CXCL9, CXCL10, CXCL11 and CXCL12) and inflammatory mediators (MMP-9, MMP-12, IL-18 and neutrophil count) were markedly increased in the serum of COPD patients compared with healthy controls. There were associations between small airway disease (E/I MLD) and CCL11, CXCL8, CXCL10, CXCL11, CXCL12 and CX3CL1. Especially CXCL8 and CX3CL1 are strongly associated with E/I MLD (r = 0.74, p < 0.001; r = 0.76, p < 0.001, respectively). CXCL8, CXCL12 and CX3CL1 were moderately positively correlated with emphysema (%LAA) (r = 0.49, p < 0.05; r = 0.51, p < 0.05; r = 0.54, p < 0.01, respectively). Bronchial wall thickness (Pi10）showed no significant differences between the COPD and healthy controls,，but there was an association between Pi10 and FEV1% in COPD patients (r=-0.420, p = 0.048). Our statistical results showed that there were not any associations between airway wall thickness (Pi10) and chemokines. CONCLUSION: Pulmonary chemokines levels are closely associated with the extent of gas trapping, small airways disease and emphysema identified on high-resolution chest CT scan. ADVANCES IN KNOWLEDGE: This study combines quantitative CT analysis with multiplex chemokines and inflammatory mediators to identify a new role of pathological changes in COPD.
OBJECTIVE: We sought to explore the relationships between multiple chemokines with spirometry, inflammatory mediators and CT findings of emphysema, small airways disease and bronchial wall thickness. METHODS: All patients with COPD (n = 65) and healthy control subjects (n = 23) underwent high-resolution CT, with image analysis determining the low attenuation area (LAA), ratio of mean lung attenuation on expiratory and inspiratory scans (E/I MLD) and bronchial wall thickness of inner perimeter of a 10-mm diameter airway (Pi10). At enrollment, subjects underwent pulmonary function studies, chemokines and inflammatory mediators measurements. RESULTS: Multiple chemokines (CCL2, CCL3, CCL5, CX3CL1, CXCL8, CXCL9, CXCL10, CXCL11 and CXCL12) and inflammatory mediators (MMP-9, MMP-12, IL-18 and neutrophil count) were markedly increased in the serum of COPD patients compared with healthy controls. There were associations between small airway disease (E/I MLD) and CCL11, CXCL8, CXCL10, CXCL11, CXCL12 and CX3CL1. Especially CXCL8 and CX3CL1 are strongly associated with E/I MLD (r = 0.74, p < 0.001; r = 0.76, p < 0.001, respectively). CXCL8, CXCL12 and CX3CL1 were moderately positively correlated with emphysema (%LAA) (r = 0.49, p < 0.05; r = 0.51, p < 0.05; r = 0.54, p < 0.01, respectively). Bronchial wall thickness (Pi10）showed no significant differences between the COPD and healthy controls,，but there was an association between Pi10 and FEV1% in COPD patients (r=-0.420, p = 0.048). Our statistical results showed that there were not any associations between airway wall thickness (Pi10) and chemokines. CONCLUSION: Pulmonary chemokines levels are closely associated with the extent of gas trapping, small airways disease and emphysema identified on high-resolution chest CT scan. ADVANCES IN KNOWLEDGE: This study combines quantitative CT analysis with multiplex chemokines and inflammatory mediators to identify a new role of pathological changes in COPD.
Authors: Carlos H Martinez; Ya-Hong Chen; Phillip M Westgate; Lyrica X Liu; Susan Murray; Jeffrey L Curtis; Barry J Make; Ella A Kazerooni; David A Lynch; Nathaniel Marchetti; George R Washko; Fernando J Martinez; Meilan K Han Journal: Thorax Date: 2012-05 Impact factor: 9.139
Authors: Eric Bradford; Sean Jacobson; Jason Varasteh; Alejandro P Comellas; Prescott Woodruff; Wanda O'Neal; Dawn L DeMeo; Xingnan Li; Victor Kim; Michael Cho; Peter J Castaldi; Craig Hersh; Edwin K Silverman; James D Crapo; Katerina Kechris; Russell P Bowler Journal: Respir Res Date: 2017-10-24
Authors: Harvey O Coxson; Asger Dirksen; Lisa D Edwards; Julie C Yates; Alvar Agusti; Per Bakke; Peter Ma Calverley; Bartolome Celli; Courtney Crim; Annelyse Duvoix; Paola Nasute Fauerbach; David A Lomas; William Macnee; Ruth J Mayer; Bruce E Miller; Nestor L Müller; Stephen I Rennard; Edwin K Silverman; Ruth Tal-Singer; Emiel Fm Wouters; Jørgen Vestbo Journal: Lancet Respir Med Date: 2013-02-01 Impact factor: 30.700